Study on decolorization process for polysaccharide from Herba Patriniae and antioxidation activity
HUI He-ping1,2, LI Xiao-dong3, SHI Yan-bin4, ZHANG Yan5, MA Jiang-peng1, CAO Ze-yu1, TANG Dong-ke1, CHANG Hong-xia1, WANG Juan-di1
1. Gansu Agriculture Technology College, Lanzhou 730020, China; 2. CAS Key Laboratory for Chemistry of Northwestern Plant Resources and Gansu Provincial Key Laboratory for Natural Medicine, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 3. Institute of Traditional Chinese Medicine, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 4. Lanzhou University, Lanzhou 730000, China; 5. Gansu University of Chinese Medicine, Lanzhou 730010, China
Abstract: Based on single factor experiment, orthogonal test method is utilized to compare the decolorization effects of H2O2 and activated carbon on crude polysaccharide from Herba Patriniae as and raw material, and the best decolorizer and decolorization process is preferred.Taking Vc as a control, DPPH· and hydroxyl radical (·OH) scavenging experiments are performed to evaluate the antioxidation activity of Herba Patriniae polysaccharides before and after decolorization.Results verify that activated carbon presents better decolorization effect.The optimum process is obtained as follows:the dosage of activated carbon is 5%, decolorization temperature remains at 65℃, and the decolorization lasts for 120 min.Under these conditions, the decolorization rate and the yield of polysaccharide are 50.21%±1.32% and 70.36%±1.25%, respectively.The obtained polysaccharide presents a certain scavenging effect on DPPH· and ·OH radicals, and decolored polysaccharide shows a stronger scavenging ability on two kinds of free radicals than undecolored polysaccharide.At low concentrations (<0.5 mg·mL-1), the ability of decolored polysaccharide to scavenge DPPH and ·OH radicals is stronger than that of Vc, and there is a positive dose-depending relationship between the concentration of polysaccharide and its scavenging ability to DPPH· and ·OH radicals.
惠和平, 李晓东, 史彦斌, 张艳, 马江鹏, 曹泽钰, 唐东科, 常红霞, 王娟弟. 败酱草多糖的脱色工艺和抗氧化活性研究[J]. 现代化工, 2021, 41(S1): 231-236,240.
HUI He-ping, LI Xiao-dong, SHI Yan-bin, ZHANG Yan, MA Jiang-peng, CAO Ze-yu, TANG Dong-ke, CHANG Hong-xia, WANG Juan-di. Study on decolorization process for polysaccharide from Herba Patriniae and antioxidation activity. Modern Chemical Industry, 2021, 41(S1): 231-236,240.
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