采用循环伏安法(cyclic voltammetry,CV)将瓜氨酸(citrulline,Cit)聚合在玻碳电极上,得到了聚瓜氨酸修饰电极(polycitrulline modified glassy carbon electrode,PCit/GCE),并对尿酸(uric acid,UA)在PCit/GCE上的电化学行为进行研究,发现UA在PCit/GCE上的氧化峰电流与裸玻碳电极相比明显增大,灵敏度明显提高。UA的氧化峰电流与其浓度呈正比,线性范围为2.0×10^-6~1.0×10^-4 mol/L,检出限为8.0×10^-7 mol/L。在测定实际样品的尿酸含量实验中,回收率为99.7%~104.1%,结果满意。

Citrulline is polymerized on a glassy carbon electrode through the cyclic voltammetry process to obtain a polycitrulline modified glassy carbon electrode (PCit/GCE).The electrochemical behaviors of uric acid on PCit/GCE are investigated.It is observed that the oxidation peak current of uric acid on PCit/GCE electrode exhibits a notable enhancement compared to that on the unmodified glassy carbon electrode,leading to a remarked improvement in sensitivity.The concentration of uric acid in the range of 2.0×10^-6-1.0×10^-4 mol·L^-1 exhibits a robust linear correlation with its oxidation peak current.Additionally,the method achieves a low detection limit of 8.0×10^-7 mol·L^-1.In the determination of actual samples,the recovery rate falls within the range of 99.7% to 104.1%,showing a satisfactory result.

朱琪(1987-),女,硕士,实验师,研究方向为电化学分析及检测。