通过自组装法制备磁性纳米复合材料金磁微粒（Fe₃O₄@Au），对其进行表征，并优化Fe₃O₄@Au模拟酶检测谷胱甘肽的体系，确定检测限、回收率和抗干扰性。研究结果表明，最佳催化体系为：H₂O₂浓度50 mmol/L，催化温度60℃，反应时间为4 min。谷胱甘肽浓度在0.01~0.5 mmol/L时，吸光度差值与浓度呈现良好的线性关系，线性方程y=1.157 5x+0.272 8，线性相关系数R²为0.990 7，最低检测限度为0.3 μmol/L，样品回收率分别为91.92%和110.31%，选择性良好。

Fe₃O₄@Au nanocomposite is prepared by self-assembling method and characterized.Fe₃O₄@Au mimetic enzyme system for detecting glutathione is optimized to determine the detection limit,recovery and anti-interference.The optimum detection conditions are obtained as follows:H₂O₂ concentration is 50 mmol·L^-1,catalytic temperature is at 60℃,and reaction time is 4 min.Under the optimized conditions,a good linear relationship is shown between the absorbance difference and the concentration of glutathione in the range of 0.01-0.5 mmol·L^-1,and the equation is y=1.157 5x+0.272 8 (R²=0.990 7).The lowest detection limit is 0.3 μmol·L^-1.The recoveries ranges from 91.92% and 110.31%.