为应对CO₂引起的温室效应，利用微藻的光合作用固定CO₂。选取栅藻、小球藻为研究对象，以BG-11培养基的氮源质量浓度（247 mg/L）为标准，分别以硝酸盐、亚硝酸盐和铵盐为培养基氮源，考察在不同氮源种类及质量浓度的培养条件下，2种微藻的生长速率、CO₂吸收速率。结果表明，在以亚硝酸钠为氮源、氮元素质量浓度为247 mg/L的培养基中，栅藻的最大比生长速率为0.040/h，固碳速率达到最高为0.036 g/（L·h）；同等条件下，小球藻的最大比生长速率为0.030/h，固碳速率达到最高为0.023 g/（L·h）。利用栅藻和小球藻固定二氧化碳是一项有效的减排技术。

In order to cope with the greenhouse effect caused by CO₂, the photosynthesis of microalgae is utilized to fix CO₂, and Scenedesmus sp.and Chlorella sp.are selected as the research objects.Taking the N source concentration of 247 mg·L^-1 for BG-11 culture medium as standard, the individual medium with different concentrations of nitrate, nitrite and ammonium salt respectively are applied to growth of microalgae to investigate the performance of the growth and CO₂ fixation rate of two kinds of microalgae.In the culture medium using NaNO₂ as N source with N concentration of 247 mg·L^-1, the maximum specific growth rate of Scenedesmus sp.is 0.040 per hour, and the CO₂ fixation rate reaches the highest 0.036 g/L/h.Under the same conditions, the maximum specific growth rate of Chlorella sp.is 0.030 per hour, and the CO₂ fixation rate reaches the highest 0.023 g/L/h.It is verified that the use of Scenedesmus and Chlorella to fix carbon dioxide is an effective emission reduction technology and has far-reaching development prospects.

马咸莹(1994-),女,硕士研究生,研究方向为预防兽医学生物材料的开发与应用,Maxianying1994@outlook.com