胰高血糖素样肽-1（GLP-1）在细胞构建过程中产生信号肽和N-2、N-22截短型等多种杂质，分离纯化难度大。以聚甲基丙烯酸酯（PMMA）、聚苯乙烯-二乙烯基苯（PS-DVB）和交联琼脂糖（AG）3种不同基质（matrix）的阴离子交换填料为研究对象，探讨填料基质、表面微观结构与层析分离效果之间的关系。研究结果表明，PMMA强阴离子交换填料对分离GLP-1具有高选择性；粒径均匀度、机械强度以及表面孔径对样品分离效果具有很大影响。

During cell construction process,glucagon-like peptide-1 (GLP-1) generates a variety of impurities such as signal peptides and N-2,N-22 truncated types,which is difficult to separate and purify.Three anion exchange packings,including polymethyl methacrylate (PMMA),polystyrene-divinylbenzene (PS-DVB) and cross-linked agarose (AG),are used as research objects.Relationships among packings matrix,surface microstructure and separation effect are discussed.Results show that PMMA packings exhibit a high selectivity for the separation of GLP-1.Particle size uniformity,mechanical strength and surface pore diameter have great influences on the separation effect.

徐灿(1996-),女,硕士研究生,研究方向为材料改性与涂层,xucanyt@163.com