在聚氨酯泡沫（PUF）固定化细胞降解氰化物研究的基础上，探讨了泡沫组分硅油（L-580）、交联剂（1006-2）以及发泡剂（水）质量分数对材料亲水性、孔壁性质的影响，并对氰化物降解菌固定化细胞量、降氰酶活的影响进行了研究。结果表明，当PUF组分中L-580、1006-2和水的质量分数分别为1%、10%、0%时制备所得泡沫用于氰降解菌的固定化，体系的总生物量比游离细胞体系提高了43%，降解氰化物的总酶活约为游离细胞体系的2倍。

Based on the previous study on cyanide degradation by PUF-immobilized cells,effects of the mass fraction changes of silicone oil (L-580),crosslinking agent (1006-2) and foaming agent (water) on the hydrophilicity and pore wall properties of polyurethane foam (PUF) are studied,and impacts on the immobilized cell amount of cyanide-degrading bacteria are investigated.Results show that the preferred mass fraction of L-580,1006-2 and water for cyanide-degrading strains immobilization shall be 1%,10% and 0%,respectively.Total biomass of PUF-immobilized cell system is 43% higher than that of planktonic cell system,and total enzyme activities in cyanide degradation doubles approximately that of planktonic cell system.

蒋郑峰(1994-),男,硕士研究生,主要研究方向为生物化工,369415855@qq.com