制备了一种新型聚合物键合硅胶亲水作用色谱（HILIC）固定相，并利用傅里叶红外分析光谱和元素分析对该固定相进行表征，结果表明，聚合物已成功固定于硅胶表面。在亲水作用色谱模式下，利用经典的核苷类化合物对上述固定相的分离性能进行评测，结果表明，所制备的固定相对核苷类样品表现出较好的分离效果，并且其保留行为符合典型的亲水作用色谱的保留特征；另外，流动相的种类和柱温对上述固定相的保留行为存在较大的影响。

In this study,a novel polymer bonded silica hydrophilic interaction chromatography (HILIC) stationary phase is synthesized.The obtained stationary phase is characterized via Fourier transform spectroscopy and elemental analysis,indicating that the novel stationary phase is prepared successfully.Under the hydrophilic interaction chromatographic mode,various classical nucleosides compounds are used to evaluate the separation performance of the stationary phase.It is found that the used analytes can be fully separated by the obtained stationary phase with a typical HILIC characteristic.In addition,the composition of mobile phase and the column temperature are found to have potential effect on the retention behavior of the obtained stationary phase material.

朱萌(1990-),女,硕士研究生,研究方向为绿色化学工艺,zhumeng19901103@163.com