建立了长春西汀原料和制剂中4种异构体的液相色谱分析方法。采用CHIRALPAK IG-3手性色谱柱，用主成分自身对照法计算异构体质量。结果表明，4种长春西汀异构体色谱峰分离良好，分离度>1.5，专属性和耐用性强，4种长春西汀异构体色谱峰面积与溶液质量浓度线性关系良好，检出限分别为0.31、0.31、0.45、0.45 ng；定量限分别为1.04、0.78、1.49、1.50 ng，相对标准偏差分别为2.53%、1.81%、1.82%、2.03%（n=6），异构体E、F、G样品加标回收率分别为99.9%、101.6%、102.0%。该方法操作简单、通用性强、测定结果准确，可用于长春西汀原料及制剂的异构体控制。

A reverse-phase HPLC method is developed for determining four isomers of vinpocetine and its raw materials.CHIRALPAK IG-3 column is adopted and the isomer contents are calculated by self-contrast method.It is shown that this method can separate four isomers well,with resolution (R)>1.5,which has good specificity and durability.The peak areas for four vinpocetine isomers all have good linear relationships with the content in solution.The detection limits for vinpocetine,isomer E,isomer F,isomer G are 0.31,0.31,0.45 and 0.45 ng,respectively,the quantitation limits are 1.04,0.78,1.49 and 1.50 ng,respectively,the recovery rates are 99.9%,101.6%,102.0% respectively and the RSD are 2.53%,1.81%,1.82% and 2.03% (n=6).This method is simple,accuracy and convenient,and can be reliable for the quality control of isomers of vinpocetine and its preparation.