以乙醇为溶剂，采用薄膜分散法制备二甲基姜黄素（ASC-J9）脂质体。通过单因素试验和正交试验优化得到最佳制备工艺：磷脂与胆固醇的质量比为10∶1、磷脂与ASC-J9的质量比为1 000∶10、水化温度为60℃以及磷脂质量浓度为6.67 mg/mL。最佳条件下制备的ASC-J9脂质体的包封率为97.99%，平均粒径为145.7 nm，其粒径分布较窄且在水中分散较好。4℃下ASC-J9脂质体的稳定性较好，且脂质体可以较好地保护包载的ASC-J9。该制备工艺具有绿色环保、操作简便、产品性能好等特点，所制ASC-J9脂质体可以有效改善ASC-J9的热稳定性和水溶性。

Dimethyl curcumin (ASC-J9) liposome is prepared by the thin film dispersion method using ethanol as solvent.The optimal preparation conditions are obtained via single factor and orthogonal experiments that the mass ratio of phospholipid to cholesterol is 10:1,the mass ratio of phospholipid to dimethyl curcumin is 1 000:10,the hydration temperature is set at 60℃ and the concentration of phospholipid is 6.67 mg·mL^-1.Under the optimal conditions,the entrapment efficiency of dimethyl curcumin liposome can reach 97.99% and its particle size is averaged at 145.7 nm,with a very narrow particle size distribution.It can be well dispersed in water and remains stable at 4℃.The liposomes can protect the entrapped dimethyl curcumin well.This preparation technology has many characteristics such as environmental friendliness,simple operation,good product performance,etc.Dimethyl curcumin liposome prepared by this technology can effectively improve the thermal stability and water solubility of dimethyl curcumin.

李正义(1979-),男,博士,副教授,研究方向为精细化工,zyli@cczu.edu.cn