建立了简便、快速、准确测定卡马西平的单波长和双波长可见吸收光谱法，探讨了反应条件、共存物质的影响及光谱特征。在弱酸性溶液中，偶氮氯膦Ⅲ与卡马西平以静电引力结合生成具有2个正吸收峰的离子缔合物，最大和次大正吸收波长分别为614 nm和666 nm，在此2个波长处，卡马西平的质量浓度在0~4.7 mg/L范围内与吸光度A呈线性关系，服从朗伯-比尔定律，表观摩尔吸光系数（κ）分别为1.58×10⁴ L/（mol·cm）（614 nm）和1.20×10⁴ L/（mol·cm）（666 nm）。用双波长叠加法测定时，其表观摩尔吸光系数（κ）达2.78×10⁴ L/（mol·cm），方法的回收率为98.0%~102%，相对标准偏差RSD（n=5）为2.3%~2.7%。该方法可用于药片中卡马西平的测定。

A simple, rapid and accurate new method for the determination of carbamazepine by single wavelength and dual-wavelength visible absorption spectra is developed.The influences brought by reaction conditions and the coexisting substances, and the spectral characteristics are discussed.In a weak acidic solution, chlorophosphonazo Ⅲ combines with carbamazepine via electrostatic attraction to form an ionic association complex with two positive absorption peaks.The maximum and secondary positive absorption wavelengths are located at 614 nm and 666 nm, respectively.At these two wavelengths, the mass concentration of carbazepine keeps linear with absorbance A in the range of 0 to 4.7 mg·L^-1, which obeys Lambert-Beer law.The apparent molar absorptivities (κ) are 1.58×10⁴ L/(mol·cm)(614 nm) and 1.20×10⁴ L/(mol·cm)(666 nm) respectively.When the dual-wavelength superposition absorption spectrophotometry is used to determine the concentrations of carbamazepine, its apparent molar absorptivity (κ) can achieve 2.78×10⁴ L/(mol·cm).The method's recovery and RSD (n=5) are in the ranges of 98.0%-102% and 2.3%-2.7% respectively.The method can be used for the determination of carbamazepine in tablets with satisfactory results.

张淑琼(1963-),女,理学学士,副教授,研究方向为分子光谱分析,zhangshuqiong2017@163.com。