采用生物还原法制备生物纳米银（Bio-Ag⁰），以透射电子显微镜和紫外-可见吸收光谱对生物纳米银形貌及在水中的稳定分散性进行表征。结果表明，所得生物纳米银颗粒形貌近球形，粒径小于10 nm，相比化学纳米银（Chem-Ag⁰），在水溶液中有更强的稳定分散性。以典型革兰氏阴性菌和革兰氏阳性菌为研究对象，对生物纳米银（Bio-Ag⁰）和化学纳米银（Chem-Ag⁰）的抗菌性能进行比较，结果表明，Bio-Ag⁰对测试细菌表现出更强的抗菌性。

Biogenic silver nanoparticles (Bio-Ag⁰) are synthesized via the bio reduction method.The morphology of Bio-Ag⁰ and its stable dispersibility in aqueous solution are characterized by means of transmission electron microscopy and ultraviolet-visible spectroscopy.The results show that the Bio-Ag⁰ nanoparticles have morphology of subglobose, with particle sizes less than 10 nm.They can well disperse in aqueous solution and exhibit stronger stability than chemically synthesized silver (Chem-Ag⁰).The antibacterial properties of Bio-Ag⁰ and Chem-Ag⁰ nanoparticles are compared by using typical Gram-negative and Gram-positive bacteria as study targets.It is found that Bio-Ag⁰ exhibits more significant antibacterial effect than Chem-Ag⁰.