微藻油脂酸值是评价微藻油脂原料的重要指标之一，对评估微藻藻粉品质以及进行微藻生物柴油的炼制具有重要参考价值。采用滴定法向微藻油脂溶液中加入饱和氯化钠溶液使溶液分层，用标准氢氧化钾乙醇溶液进行滴定，通过判断水层的颜色变化确定滴定终点，可快速准确测定深色微藻油脂的酸值。通过测定标准品混合物的酸值验证该方法的准确性。通过该方法比较了不同处理方式得到微藻藻粉样品的酸值，对藻粉品质进行了快速的评估，为考察不同处理方式对藻粉品质的影响提供了基础数据。

The acid value of microalgal oil is one of the important indexes for evaluating microalgae oil raw material,which also plays important role in evaluating the quality of microalgal powder and in refining of the microalgal biodiesel.The method for measuring quickly and accurately acid value of microalgal oil with a dark color includes steps of titrating saturated NaCl solution into the microalgal oil solution to make it form different layers,then titrating the mixture with standard ethanol solution of KOH,and distinguishing the change in color in the water layer to find the end point of titration.This method is verified by comparing the theoretical acid value of the standard mixture.This method is used to detect the acid value of microalgae powder sample acquired by different processing modes and to make a quick evaluation on the quality of microalgae powder,which supplies basic data for checking the effects of different processing modes on the quality of microalgae powder.

姜君鹏(1990-),男,硕士生,研究方向为微藻生物质能源技术,jiangjunpeng@dicp.ac.cn