Abstract: Central composite design-response surface method is utilized to optimize the extraction of chlorogenic acid,gardenoside,crocin-Ⅰ and crocin-Ⅱ by natural deep eutectic solvent.Fifteen kinds of NADESs are synthesized.On the basis of single factor experiment,the optimal extraction process is obtained and verified by CCD-RSM.Choline chloride-acetic acid is selected as the best solvent.The optimal extraction process conditions are obtained as follows:the molar ratio between choline chloride and acetic acid is 1∶2,water content is 45%,the ratio of Gardenia jasminoides Ellis powder to NADEs is 1∶15,the extraction process spends 45 min and extraction temperature remains at 55℃.Under these optimal conditions,the extraction rates of chlorogenic acid,gardenoside,crocin-Ⅰ and crocin-Ⅱ are 1.46 mg·g-1,48.44 mg·g-1,6.17 mg·g-1 and 0.81 mg·g-1,respectively.Total yield is 56.88 mg·g-1,representing a relative error of 0.58% compared with the theoretical value of 57.21 mg·g-1,which indicates that the established model is reliable.
冯智翱, 杨丹, 薄彧坤, 赵龙山, 安明. 天然低共熔溶剂提取栀子中活性成分的工艺研究[J]. 现代化工, 2022, 42(S2): 258-262,268.
FENG Zhi-ao, YANG Dan, BO Yu-kun, ZHAO Long-shan, AN Ming. Study on extraction of active ingredients from Gardenia jasminoides Ellis by natural deep eutectic solvent. Modern Chemical Industry, 2022, 42(S2): 258-262,268.
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