Abstract: The wax deposition problem happened in the process of exploitation and transportation of waxy crude oil can be solved by means of the degradation of wax components by microorganisms.Ochrobactrum intermedium H6 is screened out from the soil contaminated by crude oil in Daqing Oilfield,and the effect of strain H6 on waxy crude oil is investigated.Experimental results show that the optimal culture temperature for strain H6 is 34℃,and the optimal initial pH value of the medium is 6.Strain H6 can produce lipopeptide biosurfactants with good emulsifying properties.After it has treated with Daqing crude oil for 10 days,the wax content of crude oil drops by 5.74%.The viscosity reduction rate of crude oil by Strain H6 reaches 49.37% when the treating temperature is at 34℃,equal to the optimal culture temperature for strain.Strain H6 can degrade the wax components in crude oil,and has the potential to play a role in the process of wax removal and prevention in oil field.
何洋, 董美, 杜胜男, 李阔, 王卫强. Ochrobactrum intermedium培养条件优化及其对含蜡原油作用效果分析[J]. 现代化工, 2022, 42(S2): 196-200,204.
HE Yang, DONG Mei, DU Sheng-nan, LI Kuo, WANG Wei-qiang. Optimization of culture conditions for Ochrobactrum intermedium and analysis on its effect on waxy crude oil. Modern Chemical Industry, 2022, 42(S2): 196-200,204.
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