Influence of salt stress on bio-enhanced remediation of petroleum contaminated soil
HOU Xin-ran1, LI Qi1, LI Tian-yuan1, LI Tian2, SUN Xiao-xi3, QI Zhi-chong4, ZHANG Qiang1
1. Shandong Province Key Laboratory of Applied Microbiology, Ecology Institute of Shandong Academy of Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China; 2. Key Laboratory of Pollution Processes and Environmental Criteria of the Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; 3. Gudao Oil Production Plant, Sinopec Shengli Oilfield Branch Company, Dongying 257231, China; 4. International Joint Laboratory for Environmental Pollution Control Materials of Henan Province, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China
Abstract: The corresponding relationship between the concentrations (1%,2% and 3%) of NaCl in the soil respectively and the degrading rate of petroleum hydrocarbons in the soil,corresponding microbial quantity,microbial flora structure and dominant bacteria change are studied on the basis of adding with salt-tolerant degrading strain Staphylococcus2-24 during the remediation process.The result shows that a specific flora represents a stronger colonization ability in high salt environment (3.0% NaCl) than that in low salt environment (1% NaCl).The degrading rate of petroleum hydrocarbons,total number of culturable microorganisms and the number of degrading bacteria for petroleum hydrocarbon are all negative correlation with NaCl concentration.The abundance and diversity of microflora have little correlation with NaCl concentration in the early stage of remediation,and have a positive correlation with NaCl concentration in the late stage of restoration.Salt stress affects significantly the structure of microflora and the relative abundance of dominant bacteria.KCM-B-112,Halomonas and Virgibacillus are the dominant bacteria under salt stress.Salinity changes the bacterial community structure under the experimental conditions,and both the flora structure and the dominant bacteria change with the degradation process of petroleum hydrocarbons in the soil.
侯心然, 李琪, 李天元, 李田, 孙小喜, 祁志冲, 张强. 盐胁迫对石油污染土壤生物强化修复过程的影响[J]. 现代化工, 2022, 42(S2): 98-102,107.
HOU Xin-ran, LI Qi, LI Tian-yuan, LI Tian, SUN Xiao-xi, QI Zhi-chong, ZHANG Qiang. Influence of salt stress on bio-enhanced remediation of petroleum contaminated soil. Modern Chemical Industry, 2022, 42(S2): 98-102,107.
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