Electrochemical determination of sodium tanshinone ⅡA sulfonate and its effect on DNA
SUN Zhang-hua1, CHEN Mei-feng2, MA Xin-ying2, QIN Jia-meng2, XUE Shou-qing2
1. Shandong Heze Ecology and Environment Monitoring Center, Heze 274000, China; 2. College of Chemistry and Chemical Engineering, Heze University, Heze 274000, China
Abstract: Poly(L-leucine) modified glassy carbon electrode is prepared,and used to determine sodium tanshinone ⅡA sulfonate.The results show that poly(L-leucine) catalyzes the electrochemical redox reactions of sodium tanshinone ⅡA sulfonate on the modified electrode.The preparation and testing conditions for the electrode are optimized.The oxidation peak current of sodium tanshinone ⅡA sulfonate shows linear with its concentration.This modified electrode has good sensitivity,selectivity and stability,and can be used for the production and quality control of related products.In addition,the interaction between sodium tanshinone ⅡA sulfonate and DNA is studied by means of electrochemistry and UV spectrophotometry,respectively.It is shown that there is obvious interaction between sodium tanshinone ⅡA sulfonate and dsDNA.
孙章华, 陈美凤, 马心英, 秦佳濛, 薛守庆. 丹参酮ⅡA磺酸钠的电化学测定及与DNA作用的研究[J]. 现代化工, 2022, 42(11): 256-260.
SUN Zhang-hua, CHEN Mei-feng, MA Xin-ying, QIN Jia-meng, XUE Shou-qing. Electrochemical determination of sodium tanshinone ⅡA sulfonate and its effect on DNA. Modern Chemical Industry, 2022, 42(11): 256-260.
[1] Yu M L, Li S M, Gao X, et al.Sodium tanshinoneⅡA sulfonate for coronary heart disease:A systematic review of randomized controlled trials[J].Chin J Integr Med, 2020, 26(3):219-226. [2] 许何丽, 张新生, 谭宏友, 等.丹参酮ⅡA磺酸钠注射液对亚砷酸钠导致的H9c2心肌细胞损伤的影响[J].中国医科大学学报, 2021, 50(4), 332-335. [3] 马晓聪, 李建橡, 许明东, 等.丹参酮ⅡA磺酸钠注射液治疗不稳定型心绞痛疗效和安全性的Meta[J].世界科学技术-中医药现代化, 2015, 17(9):1766-1774. [4] Chen T T, Li M L, Fan X H, et al.Sodium tanshinoneⅡA sulfonate prevents angiotensinⅡ-induced differentiation of human atrial fibroblasts into myofibroblasts[J].Oxid Med Cell Longev, 2018, 24:1-10. [5] Hao G, Wang N, Cui J, et al.Determination of sodium tanshinone ⅡA sulfonate inplasma by liquid hromatography-electrosprayionisation-tandemmasss pectrometry[J].Biomed Chromatogr, 2007, 21:1172-1179. [6] Tan D, Wu J R, Zhang X M, et al.Sodium tanshinone ⅡA sulfonate injection as adjuvant treatment for unstable angina pectoris:A meta-analysis of 17 randomized controlled trials[J].Chin J Integr Med, 2018, 24(2):156-160. [7] Shang Q, Xu H, Huang L.Tanshinone ⅡA:A promising natural cardioprotective agent[J].Evid Based Complement Alternat Med 2012, 2012:716459. [8] Chen Z, Xu H.Anti-inflammatory and immunomodulatory mechanism of tanshinone ⅡA for atherosclerosis[J].Evid Based Complement Alternat Med, 2014:2014:267976. [9] Mao S, Jin H, Bi Y, et al.Ion-pairreversed-phase HPLC method fordetermination of sodium tanshinone ⅡA sulfonate inbiological samples andi ts pharmacokinetics and biodistributionin mice[J].Chem Pharm Bull (Tokyo), 2007, 55:753-756. [10] Mao S J, Hou S X, Liang Z, et al.Ion-pairreversed-phase HPLC:As sayvalidation ofsodium tanshinone ⅡA sulfonatein mouse plasma[J].J Chromatogr B:Anal Technol Biomed Life Sci, 2006, 831:163-168. [11] Mao S J, Jin H, Bi Y Q, et al.Ion-pair reversed-phase HPLC method for determination of sodium tanshinone ⅡA sulfonate in biological samples and its pharmacokinetics and biodistribution in mice[J].Chemical & Pharmaceutical Bulletin, 2007, 55(5):753-756. [12] Hao H, Wang G, Cui N, et al.Determination of sodium tanshinone ⅡA sulfonate inplasma by liquid chromatography-electrospray ionisation-tandem massspectrometry[J].Biomed Chromatogr, 2007, 21(11):1172-1179. [13] He T T, Zou Q G, Feng Z B, et al.Study of sodium tanshinone ⅡA sulfonate tissue distribution in rat by liquid chromatography/tandem mass spectrometry[J].Arzneimittelforschung, 2010, 60(11):660-666. [14] Qin W W, Wang B, Pei Xiao, et al.Determination of sodium tanshinone ⅡA sulfonate in human plasma by LC-MS/MS and its application to a clinical pharmacokinetic study[J].Journal of Pharmaceutical & Biomedical Analysis, 2016, 121:204-208. [15] Sunil T S, KumarNaik B E, Kumara Swamy, et al.Poly(L-leucine) modified carbon paste electrode as an electrochemical sensor for the detection of paracetamol in presence of folic acid[J].Materials Science for Energy Technologies, 2020, 3:626-632. [16] Zheng X, Guo Y, Zheng J, et al.Simultaneous determination of ascorbic acid, dopamine and uric acid using poly(L-leucine)/DNA composite film modified electrode[J].Sensors & Actuators B Chemical, 2015, 213:188-194. [17] Monnappa A B, Manjunatha J, Bhatt A S, et al.Fabrication of a sensitive and selective electrochemical sensing platform based on poly-l-leucine modified sensor for enhanced voltammetric determination of Riboflavin[J].Journal of Food Measurement and Characterization, 2020, 14(4):3633-3643. [18] Karimi-Maleh H, Tahernejad-Javazmi F, Atar N, et al.A novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug[J].Industrial & Engineering Chemistry Research, 2015, 54(14):3634-3639. [19] Chen M F, Ma X Y, Xia L, et al.Study of DNA-diethylstilboestrol interaction using graphene-based thin-film sensor and UV-Vis spectroscopy[J].Journal of the Chilean Chemical Society, 2018, 63(1):3795-3798. [20] 郭英, 贝玉祥, 高云涛.灯盏花素与DNA相互作用的电化学及吸收光谱[J].应用化学, 2009, 26(9):1045-1049.