Screening and degradation characteristics of bacteria for degrading alkane in diesel contaminated soil
WANG Xing-long, YAN Jia-bao, HU Jie, BAO Yan-zhou
Hubei Province Key Laboratory of Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China
Abstract: L12, a dominant diesel-degrading bacterium, is isolated and screened out from the contaminated soil in the diesel tank area of a refinery.After morphological observation, physiological tests, biochemical tests and 16S rDNA sequence analysis, it is identified as Acinetobacter.sp.The shake flask experiment is utilized to study the suitable degradation conditions of L12 strain to n-hexadecane, and the degradation ability of L12 strain to diesel is investigated.The optimal cultural conditions for L12 strain are obtained as follows:the initial pH is 7, the inoculum amount is 5%, the shaking speed is 150 r·min-1, and the culture temperature is at 30℃.The strain can tolerate the concentration of n-hexadecane up to 5 000 mg·L-1.If L12 strain has been cultivated for 7 days under the optimal conditions, the strain's degradation rate to n-hexadecane is as high as 90.33%, it can almost completely degrade diesel fuel with a concentration of 3 000 mg·L-1 within 7 days, indicating that the bacteria has a good potential for bioremediation of diesel polluted soil.
王兴龙, 颜家保, 胡杰, 鲍彦舟. 柴油污染土壤烷烃降解菌的筛选及降解特性研究[J]. 现代化工, 2022, 42(2): 163-166.
WANG Xing-long, YAN Jia-bao, HU Jie, BAO Yan-zhou. Screening and degradation characteristics of bacteria for degrading alkane in diesel contaminated soil. Modern Chemical Industry, 2022, 42(2): 163-166.
[1] 王智,王晓丽.原油降解菌群对石油烃的生物演化过程研究[D].天津:天津理工大学,2018. [2] 卞晓辉,马明亮,王新华,等.原油组分分离与结构鉴定[J].广州化工,2014,42(21):134-137. [3] 周楚莹.石油降解菌群的构建及其固定化技术研究[D].广州:广东工业大学,2015. [4] 陈瀑,褚小立.原油及重油的快速分析技术进展[J].分析测试学报,2012,31(9):1191-1198. [5] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001. [6] 周征西.石油污染物降解菌种鉴定及其协同作用强化研究[D].天津:天津大学,2013. [7] 高晓攀,杜显元,李兴春,等.石油降解菌处理污染土壤的研究进展[J].当代化工,2015,44(12):2814-2817. [8] 石琛,霍瑜姝,刘佳.天津大港地区石油化工企业大气污染物监测及治理方法研究[J].山东化工,2017,46(3):130-133. [9] 任丽君,刘宪斌,田胜艳.含油污泥中石油降解菌的分离及其降解特性[J].环境工程学报,2015,9(7):3538-3544. [10] 马朝阳,齐树亭,李斯,等.两株海洋原油降解菌的筛选鉴定及降解特性的初步研究[J].河北工业大学学报,2016,45(6):82-90. [11] Hu Y,Zhou J,Du B,et al.Health risks to local residents from the exposure of heavy metals around the largest copper smelter in China[J].Ecotoxicology and Environmental Safety,2019,171:329. [12] Shao B,Liu Z,Zhong H,et al.Effects of rhamnolipids on microorganism characteristics and applications in composting:A review[J].Microbiological Research,2017,200:33-44. [13] Qian C J,Wei L,Hui L,et al.Responses of genes for the uptake of glycine betaine in Virgibacillus halodenitrificans PDB-F2 under NaCl stress[J].International Biodeterioration & Biodegradation,2018,132:192-199. [14] 何丽媛,党志,唐霞,等.混合菌对原油的降解及其降解性能的研究[J].环境科学学报,2018,30(6):1220-1227. [15] 张晓娟.石油污染土壤中微生物对温度变化的响应研究[D].北京:中国地质大学,2016. [16] 李科静.石油污染对土壤的影响与对策[J].技术与市场,2016,23(11):214. [17] 余薇.石油降解微生物的筛选及其降解特性的研究[D].武汉:华中农业大学,2013. [18] 徐冯楠,冯贵颖,马雯,等.高效石油降解菌的筛选及其降解性能研究[J].生物技术通报,2010,7:221-226. [19] 刘鹏,李大平,王晓梅,等.石油污染土壤的生物修复技术研究[J].化工环保,2006,26(2):91-94. [20] 郭静波,陈微,马放,等.环境污染治理中难降解有机污染物的生物共代谢[J].安全与环境学报,2014,06:223-227.