Photothermal-chemotherapy synergistic therapy effect of molybdenum disulfide flower-like nanospheres loaded doxorubicin on cancer cells
LIAN Shuang1, LU Qi-yue1, ZHANG Yin2, XIE Wen-juan1, JIANG Dong-li1
1. Pharmacy School, Guilin Medical University, Guilin 541100, China; 2. School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China
Abstract: Flower-like MoS2 nanospheres are prepared via hydrothermal method by using sodium molybdate,thiourea,citric acid and PEG-400 as raw materials.The photothermal conversion performance of MoS2 nanospheres is studied,and the loading and controlled release ability of the nanospheres to doxorubicin (DOX) are evaluated.Photothermal-chemotherapy synergistic therapy effect of the drug delivery system on cancer cells is further explored.It is shown that the as-prepared flower-like MoS2 nanospheres with a size about 500 nm exhibit excellent photothermal effect.As a carrier,the load rate of the nanospheres to DOX can reach 136.8%,and the drug release behavior shows responsive to pH.As pH=5.8,the cumulative release amount of DOX can reach 70.29%,which is about 7 times of that at pH=7.4,indicating that the material has promising application in drug loading/controlled release.In addition,MoS2-DOX composites under near infrared laser irradiation can reach 92.09% of inhibition rate to HuH-7 liver cancer cells,which is significantly higher than that of photothermal therapy alone or chemotherapy alone.
[1] Li M,Wang D,Li J,et al.Surfactant-assisted hydrothermally synthesized MoS2 samples with controllable morphologies and structures for anthracene hydrogenation[J].Chinese J Catal,2017,38(3):597-606. [2] Dagan R,Vaknin Y,Rosenwaks Y.Gap state distribution and Fermi level pinning in monolayer to multilayer MoS2 field effect transistors[J].Nanoscale,2020,12(16):8883-8889. [3] Liu Y J,Bhattarai P,Dai Z F,et al.Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer[J].Chem Soc Rev,2019,48(7):2053-2108. [4] Wang K,Chen Q,Xue W,et al.Combined chemo-photothermal antitumor therapy using molybdenum disulfide modified with hyperbranched polyglycidyl[J].ACS Biomaterials Science Engineering,2017,3(10):2325-2335. [5] Coleman J N,Lotya M,O'neill A,et al.Two-dimensional nanosheets produced by liquid exfoliation of layered materials[J].Science,2011,331(6017):568-571. [6] Liu T,Wang C,Gu X,et al.Drug delivery with PEGylated MoS2 nano-sheets for combined photothermal and chemotherapy of cancer[J].Adv Mater,2014,26(21):3433-3440. [7] Xie J F,Zhang H,Li S,et al.Defect-rich MoS2 ultrathin nanosheets with additional active edge sites for enhanced electrocatalytic hydrogen evolution[J].Adv Mater,2013,25(40):5807-5813. [8] Tang G G,Sun J R,Wei C,et al.Synthesis and characterization of flowerlike MoS2 nanostructures through CTAB-assisted hydrothermal process[J].Mater Lett,2012,86(2012):9-12. [9] Chen L,Feng Y H,Zhou X J,et al.One-Pot synthesis of MoS2 nanoflakes with desirable degradability for photothermal cancer therapy[J].ACS Appl Mater Interfaces,2017,9(20):17347-17358. [10] Gu X D,Zheng S J,Huang X B,et al.Hybrid Ni3S2-MoS2 nanowire arrays as a pH-universal catalyst for accelerating the hydrogen evolution reaction[J].Chem Comm,2020,56(16):2471-2474. [11] Ding X G,Peng F,Zhou J,et al.Defect engineered bioactive transition metals dichalcogenides quantum dots[J].Nat Commun,2019,10(1):41. [12] Zhang K,Zhuang Y L,Zhang W D,et al.Functionalized MoS2-nanoparticles for transdermal drug delivery of atenolol[J].Drug Delivery,2020,27:909-916. [13] Xie J F,Zhang J J,Li S,et al.Controllable disorder engineering in oxygen-incorporated MoS2 ultrathin nanosheets for efficient hydrogen evolution[J].J Am Chem Soc,2013,136(47):17881-17888. [14] Feng W,Chen L,Ming Q,et al.Flower-like PEGylated MoS2 nanoflakes for near-infrared photothermal cancer therapy[J].Sci Rep,2015,5(1):17422. [15] Liu Y,Bhattarai P,Dai Z,et al.Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer[J].Chemical Society Reviews,2019,48:2053-2108. [16] Fusco L,Gazzi A,Peng G,et al.Graphene and other 2D materials:A multidisciplinary analysis to uncover the hidden potential as cancer theranostics[J].Theranostics,2020,10(12):5435-5488. [17] 秦玉杰,韩宇,靳杭,等.具有高光热转化率和环境pH响应的载药硫化铜纳米粒子[J].科学通报,2020,65(2):203-212. [18] Liu T,Wang C,Cui W,et al.Combined photothermal and photodynamic therapy delivered by PEGylated MoS2 nanosheets[J].Nanoscale,2014,7(21):9945. [19] Ma N,Yan Z H.Research progress of thermosensitive hydrogel in tumor therapeutic[J].Nanoscale Research Letters,2021,13(3):451.