Abstract: Cryogenic circulating flume is employed to simulate the environment temperature (10℃) of groundwater, and the strain comes from the activated sludge generated in aerobic tank for oily wastewater treatment in PetroChina Liaoyang Petrochemical Company.Benzene-toluene-ethylbenzene-xylene (BTEX) is taken as carbon source.Five strains, which can degrade BTEX and are resistant to low temperature, are isolated through domesticating the activated sludge, and enriching, separating and purifying bacteria.Through analyzing 16S rRNA gene and combining with morphological and physiological characteristics, DS1 and DS3 are identified as strains of Pseudomonas sp., DS2 and DS4 are identified as Bacillus sp.and DS5 belongs to Staphylococcus sp.Under low temperature conditions, DS4 is screened out as the superior bacterium with higher degradation ability.The degradation rate of o-xylene, m-xylene and p-xylene by DS4 reaches 96.41%, that of ethylbenzene, toluene and benzene is 95.38%, 80.37% and 63.92%, respectively.
张雷, 张胜楠, 周文博, 陈琦楠, 苗月. 耐低温苯系物降解菌的筛选及其效能研究[J]. 现代化工, 2021, 41(12): 83-87.
ZHANG Lei, ZHANG Sheng-nan, ZHOU Wen-bo, CHEN Qi-nan, MIAO Yue. Screening of BTEX-degrading bacteria with resistance to low temperature and study on their efficacy. Modern Chemical Industry, 2021, 41(12): 83-87.
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