Please wait a minute...
 
最新公告: 重要提醒:骗子冒充编辑部要求加作者微信,谨防上当!   关于暑假、寒假期间版面费发票及期刊样刊延迟邮寄的通知    
现代化工  2021, Vol. 41 Issue (3): 120-124,129    DOI: 10.16606/j.cnki.issn0253-4320.2021.03.024
  科研与开发 本期目录 | 过刊浏览 | 高级检索 |
一株高效原油降解菌的分离鉴定和降解特性研究
乔悦, 常世辉, 李晓涵, 王舒岚, 李大卉, 黄磊
天津理工大学化学化工学院, 天津 300384
Isolation,identification and degradation characteristics of an efficient crude oil degrading bacterium
QIAO Yue, CHANG Shi-hui, LI Xiao-han, WANG Shu-lan, LI Da-hui, HUANG Lei
School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China
下载:  PDF (3303KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 原油泄漏及盐渍化土壤的原油污染问题日益严重,高浓度原油及高盐条件下微生物活性受到抑制,生物修复技术难以广泛应用。由辽河油田原油污染土壤中分离得到1株高效原油降解菌TG-1,经鉴定为红球菌属(Rhodococcus);菌株在无机盐培养基中对6%质量分数的原油7 d降解率高达95.6%,对直链烷烃、姥鲛烷及植烷的降解率均在90%以上;在1%~6%(10~60 g/L)盐浓度下,原油降解率均在65%以上;对盐质量分数为3%及5%的原油污染土壤15 d原油降解率分别为79.3%及60.7%。结果表明,菌株TG-1表现出较好的耐盐性及高效原油降解效率,具有修复原油污染盐渍化土壤的应用潜力。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
乔悦
常世辉
李晓涵
王舒岚
李大卉
黄磊
关键词:  原油降解  红球菌  耐盐性  土壤修复    
Abstract: Crude oil leakage and crude oil polluted saline soil problems are becoming more and more serious.Microbial activity is inhibited under high-concentration crude oil and high-salt conditions,resulting in inapplicability of bioremediation technology.A high-efficiency crude oil degrading bacterium TG-1 is isolated from crude oil contaminated soil in Liaohe Oilfield,China,which is identified as Rhodococcus sp.Degradation rate of the strain to crude oil with a concentration of 6% by mass in the inorganic salt medium is as high as 95.6%.Degradation rates of linear alkanes,pristane and phytane by it all exceed 90%.Degradation rate of crude oil exceeds 65% at a salt concentration of 1%-6% (10-60 g·L-1).For the soil containing 3% and 5% of crude oil,degradation rates of crude oil by TG-1 strain can reach 79.3% and 60.7%,respectively within 15 days.It is shown that TG-1 strain exhibits good salt tolerance and high degradation efficiency to crude oil,and has the potential to repair crude oil contaminated saline soil.
Key words:  crude oil degradation    Rhodococcus    salt tolerance    soil remediation
收稿日期:  2020-05-11      修回日期:  2021-01-04          
ZTFLH:  Q89  
基金资助: 国家自然科学基金(21777113);天津市应用基础及前沿技术研究计划(15JCQNJC08800)
通讯作者:  黄磊(1980-),男,博士,副教授,研究方向为环境难降解污染物的生物治理,通讯联系人,huanglei@tjut.edu.cn。    E-mail:  huanglei@tjut.edu.cn
作者简介:  乔悦(1995-),男,硕士研究生,研究方向为原油污染的生物修复,739602699@qq.com
引用本文:    
乔悦, 常世辉, 李晓涵, 王舒岚, 李大卉, 黄磊. 一株高效原油降解菌的分离鉴定和降解特性研究[J]. 现代化工, 2021, 41(3): 120-124,129.
QIAO Yue, CHANG Shi-hui, LI Xiao-han, WANG Shu-lan, LI Da-hui, HUANG Lei. Isolation,identification and degradation characteristics of an efficient crude oil degrading bacterium. Modern Chemical Industry, 2021, 41(3): 120-124,129.
链接本文:  
https://www.xdhg.com.cn/CN/10.16606/j.cnki.issn0253-4320.2021.03.024  或          https://www.xdhg.com.cn/CN/Y2021/V41/I3/120
[1] Ali N,Dashti N,Khanafer M,et al.Bioremediation of soils saturated with spilled crude oil[J].Scientific Reports,2020,10(1):1-9.
[2] Fatima K,Imran A,Amin I,et al.Plant species affect colonization patterns and metabolic activity of associated endophytes during phytoremediation of crude oil-contaminated soil[J].Environmental Science and Pollution Research,2016,23(7):6188-6196.
[3] Kuiper I,Lagendijk E L,Bloemberg G V,et al.Rhizoremediation:A beneficial plant-microbe interaction[J].Molecular Plant-microbe Interactions,2004,17(1):6-15.
[4] Huang L,Xie J,Lv B,et al.Optimization of nutrient component for diesel oil degradation by Acinetobacter beijerinckii ZRS[J].Marine Pollution Bulletin,2013,76(1-2):325-332.
[5] Chaillan F,Le F A,Bury E,et al.Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms[J].Research in Microbiology,2004(7):587-595.
[6] Zhang Z,Gai L,Hou Z,et al.Characterization and biotechnological potential of petroleum-degrading bacteria isolated from oil-contaminated soils[J].Bioresource Technology,2010,101(21):8452-8456.
[7] Khamehchiyan M,Charkhabi A H,Tajik M.Effects of crude oil contamination on geotechnical properties of clayey and sandy soils[J].Engineering Geology,2007,89(3-4):220-229.
[8] Li P,Sun T,Stagnitti F,et al.Field-scale bioremediation of soil contaminated with crude oil[J].Environmental Engineering Science,2002,19(5):277-289.
[9] Oren A,Gurevich P,Azachi M,et al.Microbial degradation of pollutants at high salt concentrations[J].Biodegradation,1992,3(2-3):387-398.
[10] Gao Y,Wang J,Guo S,et al.Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region,China[J].Applied Soil Ecology,2015,86:165-173.
[11] Onwurah I N E,Ogugua V N,Onyike N B,et al.Crude oil spills in the environment,effects and some innovative clean-up biotechnologies[J].International Journal of Environmental Research,2007,1(4):307-320.
[12] Hua X,Wang J,Wu Z,et al.A salt tolerant Enterobacter cloacae mutant for bioaugmentation of petroleum-and salt-contaminated soil[J].Biochemical Engineering Journal,2010,49(2):201-206.
[13] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2005.
[14] Weisburg W G,Barns S M,Pelletier D A,et al.16S ribosomal DNA amplification for phylogenetic study[J].Journal of Bacteriology,1991,173(2):697-703.
[15] 黄磊,谢晶,王方梅,等.烃降解菌HL-6的分类鉴定、降解性能及应用研究[J].南开大学学报:自然科学版,2013,46(3):41-47.
[16] Roy A S,Baruah R,Borah M,et al.Bioremediation potential of native hydrocarbon degrading bacterial strains in crude oil contaminated soil under microcosm study[J].International Biodeterioration & Biodegradation,2014,94:79-89.
[17] Huang L,Liu X,Hu X,et al.Biodegradation of phenanthrene and heavy metal removal by acid-tolerant Burkholderia fungorum FM-2[J].Frontiers in Microbiology,2019,10:408.
[18] 胡鑫,李大卉,黄锦玉,等.一株烃降解菌的分离鉴定及耐盐机制[J].科学技术与工程,2019,19(25):364-373.
[19] Li C,Zhou Z X,Jia X Q,et al.Biodegradation of crude oil by a newly isolated strain Rhodococcus sp.JZX-01[J].Applied Biochemistry and Biotechnology,2013,171(7):1715-1725.
[20] Dellagnezze B M,Sousa G V,Martins L L,et al.Bioremediation potential of microorganisms derived from petroleum reservoirs[J].Marine Pollution Bulletin,2014,89(1-2):191-200.
[21] Mikolasch A,Klenk H P,Schauer F,et al.Degradation of the multiple branched alkane 2,6,10,14-tetramethyl-pentadecane (pristane) in Rhodococcus ruber and Mycobacterium neoaurum[J].International Biodeterioration & Biodegradation,2009,63(2):201-207.
[22] Kumari S,Regar R K,Manickam N,et al.Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria[J].Bioresource Technology,2018,254:174-179.
[23] Wang Z D,Fingas M F.Development of oil hydrocarbon fingerprinting and identification techniques[J].Marine Pollution Bulletin,2003,47(9-12):423-452.
[24] 李国丽,曾小英,翟立翔,等.一株石油降解菌Lysinibacillus fusiformis 23-1的筛选鉴定及原油降解特性[J].浙江农业学报,2018,30(7):1229-1236.
[25] Wang Z,Fingas M F.Development of oil hydrocarbon fingerprinting and identification techniques[J].Marine Pol-lution Bulletin,2003,47(9):423-452.
[26] Pi Y R,Chen B,Bao M T,et al.Microbial degradation of four crude oil by biosurfactant producing strain Rhodococcus sp[J].Bioresource Technology,2017,232:263-269.
[27] 付瑞敏,杨雪,谷亚楠,等.石油烃降解菌CQ6产生物表面活性剂发酵条件的优化[J].湖北农业科学,2015,54(11):2710-2714.
[28] Irene W,Marques J M,Cunha C D,et al.Identification and biodegradation potential of a novel strain of Dietzia cinnamea isolated from a petroleum-contaminated tropical soil[J].Systematic and Applied Microbiology,2007,30(4):331-339.
[29] Chen W W,Li J D,Sun X N,et al.High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3[J].International Biodeterioration & Biodegradation,2017,118:110-118.
[30] Wang K K,Hamzah A.Comparative degradation between heavy and light crude oil mediated by nitrogen-induced cell-surface hydrophobicity[J].Remediation Journal,2018,28(4):37-43.
[31] Zhao L,Deng J H,Hou H J,et al.Investigation of PAH and oil degradation along with electricity generation in soil using an enhanced plant-microbial fuel cell[J].Journal of Cleaner Production,2019,221:678-683.
[32] 黄磊,赵婷婷,贺赟,等.两株绿脓杆菌对石油污染土壤的修复作用[J].生物工程学报,2017,33(6):957-967.
[33] 宜慧,常波,杨玲引,等.高效石油烃降解菌的筛选及其对原油污染土壤的修复[J].化工环保,2018,38(4):461-465.
[1] 龙飞, 郭斌, 王欣, 王星. 微波用于石油污染土壤修复的研究进展[J]. 现代化工, 2020, 40(S1): 45-48,53.
[2] 徐杰, 刘向荣, 甄学乐, 赵顺省, 陈欣娟, 罗景雯. 响应面法优化束红球菌对DBT的脱硫条件[J]. 现代化工, 2020, 40(6): 72-77.
[3] 黄迪, 杨燕群, 肖选虎, 张振强, 陈四海, 黄志红, 肖惠宁. 土壤重金属生物有效性评价技术进展[J]. 现代化工, 2019, 39(S1): 89-94,98.
[4] 刘庆旺, 郭昊, 范振忠, 钱黎庆, 尉小明, 刘致远. 疏水缔合减阻剂的分子模拟[J]. 现代化工, 2019, 39(5): 220-223,225.
[5] 王向鹏, 郑云香, 张春晓, 姜香凝. 可适应高温含盐环境的高吸水树脂制备及性能测定[J]. 现代化工, 2018, 38(4): 144-147.
[6] 黄迪, 杨燕群, 肖选虎, 张振强, 黄志红, 肖惠宁. 土壤重金属污染治理修复剂技术[J]. 现代化工, 2018, 38(11): 39-43.
[7] 秦义, 张顶学, 刘玉莉, 程立. 缓膨耐盐性纳米复合凝胶颗粒的合成及性能研究[J]. 现代化工, 2018, 38(10): 171-174.
[8] 宋官龙. 耐温耐盐预交联凝胶颗粒的性能研究[J]. 现代化工, 2016, 36(7): 100-103.
[9] 谭蔚, 邢帅, 贡皓霜, 刘丽艳. 热碱水洗-机械脱水工艺处理石油污染土壤[J]. 现代化工, 2016, 36(6): 63-66.
[10] 马国艳, 沈一丁, 高瑞民, 郭兴, 武哲. 纳微米级丙烯酰胺微球调剖剂的应用性能研究[J]. 现代化工, 2016, 36(12): 94-96,98.
[11] 刘红梅, 褚华强, 陈家斌, 周雪飞, 张亚雷, 董林林. 过硫酸盐在地下水和土壤修复中的应用[J]. 现代化工, 2015, 35(4): 42-46.
[12] 霍立彬, 聂晶磊, 张梦莎, 徐淑民. 从化学物质角度介绍美国固体废物和土壤修复管理[J]. 现代化工, 2015, 35(1): 1-5.
[13] 薛祖源. 国内土壤污染现状、特点和一些修复浅见[J]. 现代化工, 2014, 34(10): 1-6.
No Suggested Reading articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
京ICP备09035943号-37
版权所有 © 《现代化工》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn