Abstract: Gold nanoparticles are prepared by green reduction of grape skin soaking solution.Based on the specific binding of Hg2+ to T-T mismatch in thymine (T),a novel,efficient and rapid colorimetric method for the determination of Hg2+ is established through adding thiol groups into the two ends of aptamers to modify the surface of gold nanoparticles.It is demonstrated that gold nanoparticles with good stability and uniform particle size can be obtained with 20% grape skin soak solution as the reducing agent.A red-to-blue color change can be observed in the solution when Hg2+ concentration increases.The results by UV-Vis spectrum indicate that R2 value is 0.971 1 and the working curve equation is y=-13.246 3x+0.156 6 when Hg2+ concentration locates in the range of 0.01-10 000 μmol·L-1.The detection limits of Hg2+ in deionized water and drinking water can reach as low as 4.7 μmol·L-1 and 5.4 μmol·L-1,respectively.Additionally,this colorimetric assay method exhibits excellent anti-interference performance against 5 kinds of common amino acids and 3 kinds of common metal ions.
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