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现代化工  2021, Vol. 41 Issue (2): 256-260    DOI: 10.16606/j.cnki.issn0253-4320.2021.02.048
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新型巯基化适配体修饰金纳米粒子比色检测饮用水中汞离子的研究
关桦楠, 薛悦, 刘博, 宋岩, 彭勃, 吴巧艳, 徐丽萍, 瑙阿敏
哈尔滨商业大学食品工程学院, 黑龙江 哈尔滨 150076
Colorimetric assay of mercury ions in drinking water by a novel thiolated aptamer modified gold nanoparticles
GUAN Hua-nan, XUE Yue, LIU Bo, SONG Yan, PENG Bo, WU Qiao-yan, XU Li-ping, NAO A-min
College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
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摘要 采用葡萄皮浸泡液绿色还原法制备金纳米粒子。基于Hg2+与胸腺嘧啶(T)中T-T错配部分可特异性结合的性质,在核酸适配体两端引入巯基并修饰金纳米粒子表面,进而构建出一种新型高效的快速比色检测Hg2+的方法。结果表明,以20%葡萄皮浸泡液为还原剂可以得到稳定性好且形状均一的平均粒径约为8 nm的金纳米粒子;伴随Hg2+浓度的提高,体系颜色由红色转变为蓝紫色;紫外-可见光光谱结果表明,当汞离子浓度处于0.01~10 000 μmol/L时,R2值为0.971 1,工作曲线方程为y=-13.246 3x+0.156 6;在去离子水中和饮用水中最低检测限分别为4.7、5.4 μmol/L;检测体系对5种常见氨基酸和3种常见金属离子具有明显的抗干扰性能。
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关桦楠
薛悦
刘博
宋岩
彭勃
吴巧艳
徐丽萍
瑙阿敏
关键词:  汞离子  核酸适配体  金纳米粒子  比色法  饮用水    
Abstract: Gold nanoparticles are prepared by green reduction of grape skin soaking solution.Based on the specific binding of Hg2+ to T-T mismatch in thymine (T),a novel,efficient and rapid colorimetric method for the determination of Hg2+ is established through adding thiol groups into the two ends of aptamers to modify the surface of gold nanoparticles.It is demonstrated that gold nanoparticles with good stability and uniform particle size can be obtained with 20% grape skin soak solution as the reducing agent.A red-to-blue color change can be observed in the solution when Hg2+ concentration increases.The results by UV-Vis spectrum indicate that R2 value is 0.971 1 and the working curve equation is y=-13.246 3x+0.156 6 when Hg2+ concentration locates in the range of 0.01-10 000 μmol·L-1.The detection limits of Hg2+ in deionized water and drinking water can reach as low as 4.7 μmol·L-1 and 5.4 μmol·L-1,respectively.Additionally,this colorimetric assay method exhibits excellent anti-interference performance against 5 kinds of common amino acids and 3 kinds of common metal ions.
Key words:  mercury ion    aptamer    gold nanoparticles    colorimetry    drinking water
收稿日期:  2020-04-21      修回日期:  2020-12-21           出版日期:  2021-02-20
ZTFLH:  TS254.5  
基金资助: 国家自然科学基金资助项目(31871747,31201376,31370649);黑龙江省自然科学基金项目(C2016034);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016060);中国博士后基金项目(2014T70304,2013M531009);黑龙江省博士后资助项目(LBH-Z13002);哈尔滨商业大学科研项目(17XN026);哈尔滨商业大学大学生创新创业训练计划项目(201810240080)
通讯作者:  关桦楠(1983-),男,博士,教授,研究方向为食品安全检测及生物催化分析,通讯联系人,0451-84844281,hsdghn3@163.com。    E-mail:  hsdghn3@163.com
引用本文:    
关桦楠, 薛悦, 刘博, 宋岩, 彭勃, 吴巧艳, 徐丽萍, 瑙阿敏. 新型巯基化适配体修饰金纳米粒子比色检测饮用水中汞离子的研究[J]. 现代化工, 2021, 41(2): 256-260.
GUAN Hua-nan, XUE Yue, LIU Bo, SONG Yan, PENG Bo, WU Qiao-yan, XU Li-ping, NAO A-min. Colorimetric assay of mercury ions in drinking water by a novel thiolated aptamer modified gold nanoparticles. Modern Chemical Industry, 2021, 41(2): 256-260.
链接本文:  
https://www.xdhg.com.cn/CN/10.16606/j.cnki.issn0253-4320.2021.02.048  或          https://www.xdhg.com.cn/CN/Y2021/V41/I2/256
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