To address the critical challenge of disconnection between hemostatic and antibacterial functions in trauma repair materials,this study developed a drug-loaded chitosan cryogel system that achieves synergistic enhancement of hemostasis and antimicrobial efficacy.The material features a three-dimensional interconnected macroporous network,combining ultra-high liquid absorption capacity (>5800%) with surface amino protonation.This structure facilitates rapid blood component concentration via capillary effects and mediates erythrocyte aggregation through electrostatic interactions,thereby reducing coagulation time by over 70% compared to traditional gauze.Simultaneously,levofloxacin (LEV) exhibits biphasic drug release kinetics,releasing 88.6% of the drug within 0-40 minutes,establishing a dynamic antibacterial barrier at wound sites.The initial rapid release achieves greater than 99% pathogen elimination within 40 minutes,while the sustained release phase maintains inhibition zones exceeding 43 mm for up to 24 hours.Chitosan’s electrostatic membrane disruption synergizes with LEV’s DNA gyrase inhibition mechanism to amplify antimicrobial potency.With excellent hemocompatibility,as evidenced by a hemolysis rate below 0.3%,this integrated “physical hemostasis-chemical antibacterial” strategy provides a multifunctional solution for acute trauma management,characterized by rapid responsiveness and sustained antimicrobial protection.
HuY, WangF, YeH, et al. MXene-based flexible electronic materials for wound infection detection and treatment[J]. npj Flexible Electronics, 2024, 8(1):1-15.
[2]
ZhangJ, ChenZ, ZengD, et al. Antibacterial and rapidly absorbable hemostatic sponge by aldehyde modification of natural polysaccharide[J]. Communications Materials, 2024, 5(1):1-21.
[3]
GuoB, LiangY, DongR. Physical dynamic double-network hydrogels as dressings to facilitate tissue repair[J]. Nature Protocols, 2023, 18(11):3322-3354.
[4]
YangY, SuoD, XuT, et al. Sprayable biomimetic double mask with rapid autophasing and hierarchical programming for scarless wound healing[J]. Science Advances, 2024, 10(33):eado9479.
[5]
GheorghiţăD, AntoniacI, MoldovanH, et al. Influence of lavender essential oil on the physical and antibacterial properties of chitosan sponge for hemostatic applications[J]. International Journal of Molecular Sciences, 2023, 24(22):16312.
[6]
SuY, ChenH, LiuQ, et al. Thermoresponsive gels with embedded starch microspheres for optimized antibacterial and hemostatic properties[J]. ACS Applied Materials & Interfaces, 2024, 16(10):12321-12331.
[7]
KomoriA, IriyamaH, KainohT, et al. The impact of infection complications after trauma differs according to trauma severity[J]. Scientific Reports, 2021, 11(1):13803.
[8]
OuyangX, ZhaoL, JiangF, et al. Cellulose nanocrystal/calcium alginate-based porous microspheres for rapid hemostasis and wound healing[J]. Carbohydrate Polymers, 2022, 293:119688.
[9]
MaX Y, TianL X, LiangH P. Early prevention of trauma-related infection/sepsis[J]. Military Medical Research, 2016, 3(1):33.
[10]
LauderS N, Allen-RedpathK, SlatterD A, et al. Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis[J]. Science Signaling, 2017, 10(507):eaan2787.
[11]
ZhangS, LeiX, LvY, et al. Recent advances of chitosan as a hemostatic material:Hemostatic mechanism,material design and prospective application[J]. Carbohydrate Polymers, 2024, 327:121673.
[12]
QinX, MukerabigwiJ F, MaM, et al. In situ photo-crosslinking hydrogel with rapid healing,antibacterial,and hemostatic activities[J]. e-Polymers, 2021, 21(1):606-615.
[13]
KeC L, DengF S, ChuangC Y, et al. Antimicrobial actions and applications of chitosan[J]. Polymers, 2021, 13(6):904.
[14]
YanD, LiY, LiuY, et al. Antimicrobial properties of chitosan and chitosan derivatives in the treatment of enteric infections[J]. Molecules, 2021, 26(23):7136.
[15]
WangL, PangY, XinM, et al. Evaluating the antibacterial and antibiofilm activities of chitosan derivatives containing six-membered heterocyclics against E.coli and S.aureus[J]. Colloids and Surfaces B:Biointerfaces, 2024, 242:114084.
[16]
LiJ, ZhuangS. Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria:Current state and perspectives[J]. European Polymer Journal, 2020, 138:109984.
[17]
HaiderA, KhanS, IqbalD N, et al. Advances in chitosan-based drug delivery systems:A comprehensive review for therapeutic applications[J]. European Polymer Journal, 2024, 210:112983.
[18]
SobhaniZ, SamaniS M, MontaseriH, et al. Nanoparticles of chitosan loaded ciprofloxacin:Fabrication and antimicrobial activity[J]. Advanced Pharmaceutical Bulletin, 2017, 7(3):427-432.
[19]
Wiśniewska-WronaM, KucharskaM, StruszczykM H, et al. Hemostatic,resorbable dressing of natural polymers-hemoguard[J]. Autex Research Journal, 2016, 16(1):29-34.
[20]
WeiJ, ZhangW, MouX, et al. Bioinspired hemostatic and anti-infective armor for wound healing assisted by metal-phenol-polyamine system[J]. Advanced Functional Materials, 2024, 34(4):2306267.
[21]
HuangY, BaiL, YangY, et al. Biodegradable gelatin/silver nanoparticle composite cryogel with excellent antibacterial and antibiofilm activity and hemostasis for Pseudomonas aeruginosa-infected burn wound healing[J]. Journal of Colloid and Interface Science, 2022, 608:2278-2289.
[22]
CaoS, BiZ, LiQ, et al. Shape memory and antibacterial chitosan-based cryogel with hemostasis and skin wound repair[J]. Carbohydrate Polymers, 2023, 305:120545.
[23]
RusuA, ImreS, MareA D, et al. Synthesis,characterization and antibacterial activity of a new derivative of Levofloxacin[J]. Journal of the Chilean Chemical Society, 2020, 65(3):4857-4860.
[24]
QiL, MuL, GuoX, et al. Fast expandable chitosan-fibers cryogel from ambient drying for noncompressible bleeding control and in situ tissue regeneration[J]. Advanced Functional Materials, 2023, 33(16):2212231.
PDF
(6763KB)
