A method is developed for encapsulating catalase (CAT) in terbium-based metal organic framework (Tb-MOF) materials to prepare an immobilized catalase material (CAT@Tb-MOF).The loading capacity of the immobilized catalase material reaches as high as 302.3 mg/g.The activity of catalase encapsulated is evaluated by means of UV-visible spectrophotometry and electron paramagnetic resonance spectroscopy,respectively.It is indicated that the retention rate of catalase activity exceeds 90%,and the material has good environmental adaptability and reusability.MOFs materials encapsulate catalase directly during the growth process,which has the characteristics of mild conditions and convenient operation.The catalase remains stable structure in the immobilization process,so the activity of catalase can be maintained well while the microenvironment provided by MOFs materials also ensures the environmental adaptability of the catalase.The remaining activity of catalase is 92.6% at 80℃.The activity of immobilized catalase still maintains at 90% after 10 cycles,and at about 50% after 50 days of storage.The prepared CAT@Tb-MOF has good stability,showing broad application potential in the field of industrial application.
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