利用液相脉冲放电技术提取金线吊乌龟中千金藤素并优化了工艺流程,以千金藤素的产量为响应指标,通过响应面法优化了放电电压、提取时间和液固比3种因素。结果表明,放电电压为5.5 kV、提取时间为4.2 min、液固比为52 mL/g时千金藤素的最大产量为(13.07±0.52) mg/g,与传统热浸提取产量[(6.3±0.103) mg/g]相比大幅提高;同时液相脉冲放电法的能量消耗为225 kJ/kg,低于传统热浸提取所耗能量(2041.2 kJ/kg)。

The liquid phase pulse discharge technology is utilized to extract cepharanthine from Stephania cepharantha Hayata,and the process is optimized.Taking the yield of cepharanthine as response index,three factors such as discharge voltage,extraction time and liquid-solid ratio are optimized by using response surface method.Results show that the optimal process conditions are as follows:discharge voltage is 5.5 kV,extraction time is 4.2 min,and liquid-solid ratio is 52 mL·g^-1.Under these conditions,the maximum yield of cepharanthine is 13.07±0.52 mg·g^-1,which is significantly higher than the 6.3±0.103 mg·g^-1 yield by traditional hot maceration extraction.Meanwhile,the energy consumption of the liquid pulse discharge method is 225 kJ·kg^-1,which is lower than the 2,041.2 kJ·kg^-1 of the traditional hot maceration extraction.

王毓(1999-),男,硕士生,研究方向为天然产物分离提取,13835735046@163.com;向兵(1995-),男,博士生,研究方向为天然产物分离提取,通讯联系人,825663417@qq.com。