通过恒电位还原氧化石墨烯的方法制备电化学还原氧化石墨烯修饰电极（rGO/GCE），再结合浸渍法制备出电化学还原氧化石墨烯纳米银复合修饰电极（rGO-AgNPs/GCE）。考察了螺旋霉素（SPY）在rGO-AgNPs/GCE上的电化学响应情况，并对修饰量、电还原时间、浸渍时间、支持电解质种类及酸碱度等实验条件进行优化。结果显示，在2.0×10^-6~1.0×10^-4 mol/L浓度范围内，SPY氧化峰电流与其浓度呈显著的线性关系，线性方程为I_p=0.528 5c+26.085，r=0.997 3，检测下限为4.0×10^-7 mol/L。稳定性、可重复性和回收率实验取得令人满意的结果。

Electrochemical-reduced graphene oxide modified electrode (rGO/GCE) is prepared through potentiostatic reduction of graphene oxide, and used to prepare electrochemical-reduced graphene oxide-nano silver composite modified electrode (rGO-AgNPs/GCE) by impregnation method.The electrochemical response of spiramycin on rGO-AgNPs/GCE is investigated, and a series of experimental conditions are optimized, including the amount of modification, impregnation time, electric reduction time, type and pH of supporting electrolyte.It is shown that the oxidation peak current of spiramycin has a significant linear relationship with its concentration in the range of 2.0×10^-6-1.0×10^-4 mol·L^-1, the linear equation is I_p=0.528 5c+26.085, and the correlation coefficient is 0.997 3, and the lowest detection limit is 4.0×10^-7 mol·L^-1.It also shows a satisfied result in stability, repeatability and recovery.