在Clark-Lubs缓冲溶液中，基于酒石酸泰乐菌素与阳离子甲基紫试剂发生电荷转移反应，建立了三波长叠加共振光散射法（TRLS）测定酒石酸泰乐菌素含量的新方法。研究发现，在实验条件下，共振光散射图谱中会产生3个特征散射峰，分别在322、510、628 nm。在322、510、628 nm处，酒石酸泰乐菌素的质量浓度都在0.04~0.2 mg/L内呈线性关系；相关系数分别为0.999 4、0.999 5、0.999 2；检出限分别为0.036、0.040、0.038 mg/L。而采用三波长叠加法测定时，酒石酸泰乐菌素的质量浓度在0.04~0.2 mg/L内呈线性关系，相关系数为0.999 9，检出限为0.013 mg/L，检出限为单波长检测法的3倍。因建立的TRLS新方法简便、省时、灵敏度高，故可用于酒石酸泰乐菌素含量的大批量检测。

In Clark-Lubs buffer solution,a novel three-wavelength superimposed resonance light scattering (TRLS) method for measuring the content of tylosin tartrate is established based on the charge transfer reaction between tylosin tartrate and cationic methyl violet reagent.It is found that under experimental conditions,three characteristic scattering peaks will appear in the resonance light scattering spectrum at 322 nm,520 nm and 628 nm,respectively.At 322 nm,510 nm,and 628 nm,the mass concentration of tylosin tartrate shows linear relationship within 0.04-0.2 mg·L^-1,with correlation coefficients of 0.999 4,0.999 5,and 0.999 2,respectively,and detection limits of 0.036 mg·L^-1,0.040 mg·L^-1 and 0.038 mg·L^-1,respectively when single-wavelength detection method is employed.When three-wavelength superimposed method is employed,the mass concentration of tylosin tartrate shows a linear relationship within the range of 0.04-0.2 mg·L^-1,with a correlation coefficient of 0.999 9,and a detection limit of 0.013 mg·L^-1 that is one third that by single-wavelength detection method.The newly established TRLS method is simple,time-saving and highly sensitive,so it can be used for mass detection of tylosin tartrate content.

袁莉(1998-),女,硕士生