制备了多壁碳纳米管/聚甲基红膜修饰电极（MWCNTs/PMR-GCE），采用循环伏安法（CV）和差分脉冲伏安法（DPV）研究了替米考星（TMS）在修饰电极上的电化学行为。研究了修饰量、缓冲体系、最佳pH、扫描速率对TMS检测效果的影响。结果表明，在柠檬酸-Na₂HPO₄（pH=6）的缓冲溶液中，TMS在修饰电极上出现了不可逆氧化峰。在4.0×10^-6~1.0×10^-4 mol/L的浓度范围内，TMS的氧化峰电流与TMS浓度呈良好的线性关系，线性方程为I_p=0.143 2C+6.973，相关系数r=0.995 8，最低检出限为6.0×10^-7 mol/L。所制备的修饰电极具有良好的稳定性和可重复性。研究表明，MWCNTs/PMR-GCE可用于替米考星的电化学检测。

Multi-walled carbon nanotube/polymethyl red film modified electrode (MWCNTs/PMR-GCE) is prepared.The electrochemical behavior of tilmicosin on the modified electrode is studied by means of cyclic voltammetry (CV) and differential pulse voltammetry (DPV).The influences of modification amount,buffer system,the best pH value and scan speed on the detection effect of tilmicosin are also investigated.It is indicated by results that an irreversible oxidation peak of tilmicosin is observed on the modified electrode in citric acid-Na₂HPO₄ (pH=6) buffer solution.The oxidation peak current of tilmicosin shows a good linear relationship with its concentration in the range of 4.0×10^-6 mol·L^-1 to 1.0×10^-4 mol·L^-1,and the linear equation is:I_p=0.143 2C+6.973,r=0.995 8,and the detection limit is 6.0×10^-7 mol·L^-1.The prepared electrode has good stability and repeatability.It is shown that MWCNTs/PMR-GCE can be used to the electrochemical detection of tilmicosin.

秦洪伟(1983-),男,博士,副教授,研究方向为纳米修饰电极的制备和环境污染物及其毒性电化学检测方法研究,qinhw037@163.com