为揭示适冷假单胞菌1-2D菌株降解菲的机理,对该菌株的菲降解底物广谱性、代谢产物及酶动力学进行了研究。结果表明,该菌株具有降解广谱性,可利用羧酸类、酚类和杂环化合物为唯一碳源进行代谢。菲代谢中间产物有1-羟基-2-萘甲酸、1-萘酚、水杨酸、邻苯二酚和β-胡萝卜素,初步推测菌株1-2D的菲降解过程存在水杨酸途径和邻苯二甲酸途径。此外,菌株1-2D代谢菲的酶动力学参数V_max为217 nmol/(min·mg)、K_m为1.61 μmol/L。

In order to reveal the degradation mechanism of phenanthrene by Pseudomonas aeromonas 1-2D strain,the phenanthrene degradation substrates broad spectrum,metabolites and enzyme kinetics of the strain are studied and analyzed.The results indicate that the strain has a broad degradation spectrum and can metabolize carboxylic acids,phenols or heterocyclic compounds as only carbon source.The intermediate metabolites of phenanthrene include 1-hydroxy-2-naphthol,1-naphthol,salicylic acid,catechol and β-carotene.It is preliminarily speculated there exist salicylic acid and phthalic acid pathways in the phenanthrene degradation process by strain 1-2D.In addition,V_max and K_m,the enzyme kinetic parameters of phenylene metabolism in strain 1-2D,are 217 nmol/(min·mg) and 1.61 μmol/L,respectively.