为进一步提高前期筛选获得的Alcaligenes faecalis CL-8菌株的硝化效率,对该菌株在硝化过程中的发酵条件进行优化,通过对碳源种类、碳源浓度、初始pH、培养温度、摇床转速进行单因素实验,确定了Alcaligenes faecalis CL-8的最适培养条件为:乙酸钠为碳源、C/N=10、培养温度为28℃、摇床转速为160 r/min、pH=7.2。实验证明钙离子的存在会对菌株CL-8产生抑制作用,钙离子浓度越高抑制作用越强。将菌株投入废水中进行污水处理实验,结果表明,Alcaligenes faecalis CL-8对废水处理有积极作用,且能够在高氨氮废水处理中占据种群优势。

Alcaligenes faecalis CL-8,an efficient nitrifying strain,is preliminarily screened out from active sludge.In order to improve the nitrifying efficiency of the strain,the fermentation conditions of the strain in the nitrification process are optimized.Through single factor experiments on carbon source type,carbon source concentration,initial pH,culture temperature and shaking table speed,the optimum culture conditions for alcaligenes faecalis CL-8 are determined as follows:sodium acetate is used as carbon source,C/N=10,culture temperature is at 28℃,pH=7.2,and the shaking table speed is 160 rpm.It is proved that the presence of calcium ion has an inhibitory effect on CL-8 strain,and the higher the calcium ion concentration,the stronger the inhibitory effect.Alcaligenes faecalis CL-8 is proved to have a positive effect on wastewater treatment,and is able to dominate the population in the initial stage of inoculation.