以糖醇螯合钙为研究对象，建立了分离纯化与螯合率测定方法。研究了振荡、离心、超声等提取手段对丙酮分离提纯糖醇螯合钙效果的影响，比较了电导率法、分光光度法和乙二胺四乙酸（EDTA）配位滴定法测定中心离子螯合率的差异。结果表明，振荡提取效果优于离心和超声提取，最佳条件为振荡频率300 r/min、反应时间30 min，提取率大于99%；电导率法测定螯合率误差范围为0.22%~0.44%，重复性良好；分光光度法误差范围为1.27%~3.11%；EDTA配位滴定法误差为1%左右；3种方法均可用于糖醇螯合钙的螯合率测定，其中电导率法和EDTA配位滴定法为首选方法。

The separation, purification and chelation rate determination methods for sugar alcohol chelated calcium are established.Influences of oscillation, centrifugation and ultrasonic extraction measurements on the separation and purification of sugar alcohol-chelated calcium by acetone are studied.The conductivity method, spectrophotometry, and ethylenediamine tetra acetic acid (EDTA) coordination titration methods are compared in determining the center ion chelating rate.Results show that oscillation extraction presents a better effect than centrifugation extraction and ultrasonic extraction.The extraction rate exceeds 99% after 30 min of reaction under the optimum oscillation frequency of 300 r·min^-1.The error of chelating rate determined by conductivity method is less than 1%, and the repeatability is good.The error range by spectrophotometry is 1.27%-3.11%.The error by EDTA coordination titration is around 1%.Three kinds of determination methods all can be used to determine the chelating rate of sugar alcohol chelated calcium.

崔玉照(1997-),男,硕士生。