采用离子液体协同超高压微射流技术对雨生红球藻中虾青素（AXT）进行提取，并利用单因素实验及BOX-Behnken响应面法对离子液体添加量、提取时间、提取压力、固液比提取工艺进行优化，得到最佳条件为：离子液体添加量为45 mL、提取压力为96 MPa、提取时间为110 s、固液比为1：650，在此条件下AXT提取率达（81.66±0.17）%，比传统的超高压微射流法提取率提高了（18.93±0.78）%。抗氧化活性测定结果表明，AXT具有较好的抗氧化性。

Astaxanthin is extracted from haematococcus pluvialis through synergistically using ionic liquid and ultra-high pressure microjet technology.Single factor experiment and Box-Behnken response surface method are used to optimize the extraction process.The optimal conditions are obtained as follows:the addition amount of ionic liquid is 45 mL, extraction pressure is 96 MPa, extraction time is 110 s and the ratio of solid-liquid is 1:650.Under these conditions, the extraction yield of astaxanthin reaches 81.660.17%, which is 18.930.78% higher than that with traditional ultra-high pressure microjet method.The astaxanthin extracted is determined, showing that it has a good antioxidant activity.

高红(1996-),女,硕士研究生,研究方向为天然产物分离与提取,1829560276@qq.com。