从长庆油田安塞地区的含油土壤中筛选得到1株高效石油烃降解酵母菌，对其进行了生理、生化以及ITS序列分析，并研究了其降解特性和对原油四组分的降解情况。结果表明，筛选得到的目标酵母菌Y4为水生假丝酵母（Candida aquaetextoris），通过对降解条件进行响应面优化研究，得到该菌最佳降解条件为：pH为7.2、温度为30℃、接种量为6.25%，在该条件下，酵母菌Y4具有较好的石油烃降解能力，在石油污染修复领域具有巨大的应用潜力。

A high-efficient yeast strain for degrading petroleum hydrocarbon is screened out from the oil-bearing soil in Ansai area of Changqing Oilfield.Its physiological characteristics,biochemical characteristics and ITS sequence are analyzed,and its degradation characteristics and its degradation performance against four components of crude oil are studied.It is shown that the target yeast Y4 is Candida aquaetextoris.Through response surface optimization to the degradation conditions,the optimal degradation conditions are obtained as follows:pH=7.2,temperature is at 30℃ and inoculum amount is 6.25%.Under these conditions,the yeast Y4 has a good ability to degrade petroleum hydrocarbons and has great potential in the field of petroleum pollution remediation.

韩宇星(1996-),女,硕士研究生,主要从事环境污染的生物与化学防治研究,17729506790@163.com