通过正交实验和单因素实验对巨大芽孢杆菌MSP03产耐有机溶剂蛋白酶的培养基配方和发酵产酶条件进行优化。得到最佳发酵培养基配方为：糊精3 g/L，豆粕粉1 g/L，K₂HPO₄ 0.1 g/L。发酵条件为：装液量70 mL/250 mL，接种量5%，初始pH 7.0，于25℃发酵36 h。通过优化，耐有机溶剂蛋白酶活性增加到248.65 U/mL，是优化前的1.7倍，具有潜在的工业应用价值。

The culture medium formula and fermentation conditions for the production of organic solvent-stable protease by Bacillus megaterium MSP03 are optimized through orthogonal experiment and single factor experiment.The optimal fermentation medium formula are obtained as follows:3 g·L^-1 of dextrin, 1 g·L^-1 of soybean meal powder and 0.1 g·L^-1 of K₂HPO₄.The optimal fermentation conditions are determined as follows:the liquid volume is 70 mL/250 mL, the inoculation volume is 5%, the initial pH is 7.0, and the fermentation has been performed at 25℃ for 36 h.Through optimization, the activity of organic solvent-stable protease increases to 248.65 U·mL^-1, which is 1.7 times that before optimization, and has a potential industrial application value.

陈健(1977-),男,本科,高级工程师,主要从事食品及食品相关产品检验及实验室管理工作