以盐酸多巴胺在碱性条件下氧化自聚形成的PDA微球为载体，原位吸附丁香酚制备聚多巴胺负载丁香酚（EO@PDA）微球。通过纳米粒度仪、扫描电子显微镜、FT-IR和UV-Vis等对EO@PDA微球的形貌、粒径以及释放行为进行研究。结果表明，EO@PDA微球呈规整球形，粒径在272~866 nm之间可控，丁香酚平衡负载量为0.295 mg/mg，释放符合Higuchi模型，在24 h内累积释放率达到74.62%。抑菌实验结果表明，EO@PDA微球对金黄色葡萄球菌（S.aure）的最小抑菌质量浓度（MIC）和最小杀菌质量浓度（MBC）分别为16.5 mg/mL和66 mg/mL；对大肠杆菌（E.coli）的MIC和MBC分别为33 mg/mL和132 mg/mL；与游离丁香酚相比，EO@PDA微球作用于金黄色葡萄球菌24 h的抑菌率提高了46.02%。因此，丁香酚经PDA微球负载后具有良好的缓释性能和长效抑菌作用。

Polydopamine supported eugenol (EO@PDA) microspheres are prepared by loading eugenol in situ to polydopamine (PDA) microspheres obtained by oxidative polymerization of dopamine hydrochloride in alkaline condition.The structure, morphology, and release kinetics of EO@PDA microspheres are analyzed by nanoparticle size analyzer, scanning electron microscope, FT-IR and UV-Vis.The results show that EO@PDA microspheres show regular sphere with a controllable particle diameter from 272 nm to 866 nm.The equilibrium loading amount of eugenol is 0.295 mg·mg^-1, the release of eugenol is consistent with Higuchi model, and the cumulative release of eugenol reaches 74.62% within 24 hours.Antibacterial experiments show that the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of EO@PDA microspheres against Staphylococcus aureus are 16.5 mg·mL^-1 and 66 mg·mL^-1, respectively;MIC and MBC against Escherichia coli are 33 mg·mL^-1 and 132 mg·mL^-1, respectively.Compared with free eugenol, the inhibition rate of EO@PDA microspheres to S·aure increases by 46.02% within 24 hours.These results indicate that eugenol loaded by PDA microspheres has good sustained-release properties and long-term antibacterial effect.

陈茹茹(1995-),女,硕士研究生,研究方向为高分子微球及微胶囊的制备及性能研究,1242161275@qq.com