采用葡萄皮浸泡液绿色还原法制备金纳米粒子。基于Hg²⁺与胸腺嘧啶（T）中T-T错配部分可特异性结合的性质，在核酸适配体两端引入巯基并修饰金纳米粒子表面，进而构建出一种新型高效的快速比色检测Hg²⁺的方法。结果表明，以20%葡萄皮浸泡液为还原剂可以得到稳定性好且形状均一的平均粒径约为8 nm的金纳米粒子；伴随Hg²⁺浓度的提高，体系颜色由红色转变为蓝紫色；紫外-可见光光谱结果表明，当汞离子浓度处于0.01~10 000 μmol/L时，R²值为0.971 1，工作曲线方程为y=-13.246 3x+0.156 6；在去离子水中和饮用水中最低检测限分别为4.7、5.4 μmol/L；检测体系对5种常见氨基酸和3种常见金属离子具有明显的抗干扰性能。

Gold nanoparticles are prepared by green reduction of grape skin soaking solution.Based on the specific binding of Hg²⁺ to T-T mismatch in thymine (T),a novel,efficient and rapid colorimetric method for the determination of Hg²⁺ is established through adding thiol groups into the two ends of aptamers to modify the surface of gold nanoparticles.It is demonstrated that gold nanoparticles with good stability and uniform particle size can be obtained with 20% grape skin soak solution as the reducing agent.A red-to-blue color change can be observed in the solution when Hg²⁺ concentration increases.The results by UV-Vis spectrum indicate that R² value is 0.971 1 and the working curve equation is y=-13.246 3x+0.156 6 when Hg²⁺ concentration locates in the range of 0.01-10 000 μmol·L^-1.The detection limits of Hg²⁺ in deionized water and drinking water can reach as low as 4.7 μmol·L^-1 and 5.4 μmol·L^-1,respectively.Additionally,this colorimetric assay method exhibits excellent anti-interference performance against 5 kinds of common amino acids and 3 kinds of common metal ions.