建立了快速、准确检测药物中吡哌酸的高灵敏共振瑞利散射法。在pH为9.64的碱性Tris-HCl介质中，吡哌酸与碱性艳蓝B相互作用生成二元离子缔合物，使共振瑞利散射（RRS）信号显著增强，并产生新的RRS光谱。最大共振瑞利散射峰位于365 nm，吡哌酸的质量浓度在0.007~0.55 mg/L范围内与体系的共振瑞利散射增强强度（ΔI_RRS）呈线性关系，检出限为0.006 5 mg/L。该方法用于药物中吡哌酸的测定，加标回收率为98.2%~103%，相对标准偏差（n=5）为2.0%~2.5%。

A rapid,accurate,highly sensitive and new resonance Rayleigh scattering method for determination of pipemidic acid in drugs is established.In an alkaline Tris-HCl medium with a pH value of 9.64,the interaction between pipemidic acid and basic brilliant blue B can form a binary ionic associate complex,which distinctly enhances resonance Rayleigh scattering (RRS) signal of system and comes into being a new resonance Rayleigh scattering spectrum.The maximum resonance Rayleigh scattering peak locates at 365 nm where the mass concentration of pipemidic acid in the range of 0.007 to 0.55 mg·L^-1 shows a good linear relationship with the enhanced intensity (ΔI_RRS) of resonance Rayleigh scattering,and the detection limit is 0.006 5 mg·L^-1.As this method is applied to the determination of pipemidic acid in drugs,the spiked recovery rate is 98.2%-103% and the relative standard deviation RSD (n=5) is 2.0%-2.5%.

李莉娟(1998-),女,本科,研究方向为分子光谱分析,Lilijuan20182019@163.com