通过罗丹明B与氨曲南的荧光反应建立了测定药物及生物样品中氨曲南的荧光分析新方法。在pH 6.55的弱酸性介质中，罗丹明B与氨曲南反应生成红色离子缔合物，使体系发生荧光猝灭，氨曲南在0.015~17.4 mg/L范围内与缔合物的荧光猝灭程度（ΔF）呈线性关系，检出限（3S_b/S）为0.010 mg/L。同时，还考察了荧光光谱特征、适宜反应条件及共存物质的影响。结果表明，该方法可用于药物及人体尿液中氨曲南含量的测定，加标回收率为97.7%~103%，相对标准偏差（RSD）（n=5）为2.0%~2.8%。

The fluorescence reaction between rhodamine B and aztreonam is studied and a novel sensitive fluoroscopy for the determination of aztreonam in drugs and biological samples is established accordingly.In a weak acidic medium with pH=6.55,rhodamine B reacts with aztreonam to form a red ionic association complex,which leads to the fluorescence quenching of the system.The mass concentration of aztreonam in the range of 0.015-17.4 mg·L^-1 shows a direct proportion to the fluorescence quenching degree(ΔF) of system,with a detection limits (3S_b/S) of 0.010 mg·L^-1.The spectral characteristics of fluorescence,the suitable reaction conditions and the effect of coexisting substances are also studied.The results show that this method can be applied to determining the content of aztreonam in the drugs and human urine,with a spiked recovery in the ranges of 97.7%-103% and RSD(n=5) in the range of 2.0%-2.8%.

程乙真(1995-),男,本科,研究方向为分子光谱分析,chengyiyi2017@163.com