探讨了玫瑰精B与赖诺普利的相互作用，建立了测定药物中赖诺普利的荧光分析新方法。在λ_ex/λ_em=556 nm/585 nm、pH 7.50 Tris-盐酸条件下，玫瑰精B与赖诺普利反应生成二元离子缔合物，使体系的荧光强度显著增强，赖诺普利的质量浓度在0.06~4.1 mg/L范围内与体系的荧光增强强度（ΔF）呈线性关系，检出限（3S_b/S）为0.009 2 mg/L。该方法可用于市售赖诺普利片剂和胶囊中赖诺普利的测定，回收率为98.7%~103%，相对标准偏差RSD（n=6）为2.2%~2.8%。

The interaction action between rhodamine B and lisinopril is discussed and a novel fluorescence analysis method for determination of lisinopril in drugs is established.Under the conditions of λ_ex/λ_em=556 nm/585 nm and Tris-hydrochloric acid of pH 7.50,rhodamine B reacts with lisinopril to form a binary ion association complexes,which enhances distinctly the fluorescence intensity of the system.The mass concentration of lisinopril in the range of 0.06 to 4.1 mg·L^-1 is directly proportional to fluorescence enhancement intensity (ΔF) of the system,with a detection limit (3S_b/S) of 0.009 2 mg·L^-1.This method can be applied to the determination of lisinopril in commercially available lisinopril tablets and capsules,with recovery between 98.7% and 103%,and the relative standard deviations RSD (n=6) in the range of 2.2%-2.8%.

何树华(1971-),女,理学学士,教授,研究方向为分子光谱分析,heshuhua20182019@163.com