由于透明质酸（hyaluronic acid，HA）水凝胶的机械强度较弱，使其应用受到一定限制。以HA为原料、1，4-丁二醇二缩水甘油醚（1，4-butanediol diglycidyl ether，BDDE）为交联剂对HA进行交联制备HA凝胶，并在交联的同时用浸泡法在凝胶内复合碳酸钙（CaCO₃），以期形成力学性能优异的HA-CaCO₃复合凝胶。利用扫描电镜、红外光谱仪、热重分析仪和X射线衍射仪等对HA凝胶和HA-CaCO₃复合凝胶进行表征和分析，考察CaCO₃对HA凝胶的影响。结果表明，该方法制备的HA-CaCO₃复合凝胶的断裂伸长率达到401%，是单纯HA凝胶的2.73倍。HA-CaCO₃复合凝胶中CaCO₃的质量分数为27.3%，晶型为方解石，尺寸在30~80 nm，达到了纳米级别。HA-CaCO₃复合凝胶以其优异的力学性能进一步拓展了HA类凝胶的应用。

Hyaluronic acid (HA) hydrogel is an ideal biomedical material due to its biocompatibility,biodegradability,biological activity and no immunogenicity.However,the application of HA hydrogel has been limited due to its weak mechanical strength.Herein,1,4-butanediol diglycidyl ether (BDDE) is employed as a crosslinking agent for HA to prepare HA hydrogel.While it is cross linking,CaCO₃ is composited into HA hydrogel through soaking method for the purpose of fabricating HA-CaCO₃ composite hydrogel with excellent mechanical properties.The properties of HA hydrogel and HA-CaCO₃ nanocomposite hydrogel are characterized by scanning electron microscope,infrared spectrometer,thermo gravimetric analyzer and X ray diffractometer to investigate the influence of CaCO₃ on HA hydrogel.The results indicate that the elongation at break of composite hydrogel reaches 401%,2.73 times that of HA hydrogel.The mass fraction of CaCO₃ in composite hydrogel is 27.3%,CaCO₃ exhibits calcite crystal form with sizes between 30-80 nm.Hence,by means its excellent mechanical properties,HA-CaCO₃ composite hydrogel is expected to further extend the application scope of HA hydrogel.

华晓斌(1993-),男,硕士研究生,研究方向为生物医用材料,lsschxb@qq.com。