The technology for removing total nitrogen by "deep bed denitrification biological filter+special microorganism" is introduced, and is studied through pilot test in treating with e petrochemical wastewater.The test results reveal that this technology can remove effectively total nitrogen pollutants in the wastewater with a removal rate over 80% and total nitrogen in the effluent less than 15 mg·L-1.The quality of water after treatment can meet the latest discharge standard.