为了测定温泉水中硒的质量浓度，以邻苯二胺为显色剂，采用紫外可见分光光度法测定里松温泉源头水中硒的质量浓度。实验结果表明，体系测定波长为333 nm，硒质量浓度在0.05~0.40 μg/mL范围内具有良好的线性关系，其线性方程为y=1.598x+0.099 5，相关系数为0.999 5，回收率为98.5%~101.6%，体系在2 h内稳定。该方法操作简便、准确率高，可用于温泉水中硒质量浓度的测定。

The concentration of selenium in the source water of Lisong hot spring is determined by UV-visible spectrophotometry with o-phenylenediamine as color reagent.The results show that the detection wavelength for the system is 333 nm,and selenium content exhibits a good linear relationship in the range of 0.05-0.40 μg·mL^-1,with a linear equation of y=1.598x+0.099 5 and a correlation coefficient of 0.999 5.The recovery rate remains in the range of 98.5%-101.6%.The system can keep stable within 2 h.This method features with simple and high accuracy,and can be applied for the determination of selenium content in hot spring water.

陈秋娟(1983-),女,硕士,讲师,研究方向为天然产物提取、纯化与分析,2002090099@163.com