利用自行设计和制造的微流控两相和三相装置实现对聚乳酸-羟基乙酸共聚物[poly（lactic-co-glycolic acid），PLGA]微球的可控制备，并对其性能进行研究，调控微流控操作参数，制备PLGA微球，并以传统乳化/溶剂挥发法作对照同时制备PLGA微球，对所得微球的形貌及粒径进行分析。结果表明，乳化/溶剂挥发法得到的微球分散性差，大小不均一，离散系数（coefficient of variation，CV）为44.13%，而微流控法得到的微球分散性极好，大小均一，且CV仅为2.73%（两相）和1.93%（三相），说明微流控法能够制备单分散性很高的PLGA微球，有着乳化/溶剂挥发法无法比拟的优势。

The controllable preparation of poly(lactic-co-glycolic acid) (PLGA) microspheres is achieved by self-designed and self-made two-fluid-channel device and three-fluid-channel microfluidics device.The performance of PLGA microspheres is studied.The microfluidics parameters are adjusting and varying in the preparation process.PLGA microspheres are also prepared by traditional emulsion/solvent evaporation method,and are taken as a reference.Morphology and size distribution of the PLGA microspheres prepared by both methods are investigated.It is shown that PLGA microspheres prepared by emulsion/solvent evaporation method has serious aggregation and a wide size distribution with a coefficient of variation (CV) being 44.13%,whereas those prepared by two-fluid-channel microfluidics device and three-fluid-channel microfluidics device both have good dispersive performance,narrow size distribution,and CV of 2.73% (two-fluid-channel device) and 1.93% (three-fluid-channel device),respectively.It indicates that using microfluidics can manufacture highly mono-dispersive PLGA microspheres without aggregation.Hence,it is possible to achieve the precise control over the preparation of PLGA microspheres.

陈红(1992-),女,硕士,研究方向为生物医用材料,1252327912@qq.com