在pH为3.87的Tris-盐酸介质中，Cu（Ⅱ）与固绿FCF在溴代十六烷基吡啶（TPB）存在下结合生成离子缔合物，使体系的瑞利光散射（RLS）信号显著增强并产生新的瑞利散射光谱，最大瑞利散射峰位于366 nm波长处，Cu（Ⅱ）的质量浓度在0.01~0.22 mg/L范围内与体系的瑞利散射增强程度（ΔI_RLS）呈线性关系，检出限为0.009 5 mg/L，定量限为0.013 mg/L。由此建立了高灵敏、快速准确测定Cu（Ⅱ）的瑞利散射新方法，还研究了体系的瑞利散射光谱特征及适宜反应条件。该方法的加标回收率为98.0%~101%，相对标准偏差RSD（n=5）为1.8%~2.4%，适于市售饮料中Cu的测定。

In a tris-hydrochloric acid medium of pH 3.87 and in the presence of cetylpyridine bromide (CPB), Cu(Ⅱ) combines with fast green FCF to form ionic association complexes, which distinctly enhances Rayleigh light scattering (RLS) signal of the system and generates a new Rayleigh light scattering spectrum. The maximum Rayleigh scattering peak is located at 366 nm. The mass concentration of copper(Ⅱ) in the range of 0.01 to 0.22 mg·L^-1 has linear relationship to the Rayleigh scattering enhanced intensity (ΔI_RLS) of system with the detection limits at 0.009 5 mg·L^-1 and the quantitative limit at 0.013 mg·L^-1. Based on the results, a highly sensitive, rapid, accurate and new Rayleigh light scattering method for detecting Cu(Ⅱ) is established. The spectrum characteristics of Rayleigh light scattering of the system and the suitable reaction conditions are discussed. The standard addition recovery rate and RSD (n=5) of this method are in the range of 98.0%-101% and 1.8%-2.4% respectively. This method is suitable for the determination of copper in beverages in the market.

张淑琼(1963-),女,理学学士,副教授,研究方向为分子光谱分析,zhangshuqiong2017@163.com。