建立了QuEChERS提取和净化样品的改进方法，利用超高效液相色谱-三重四级杆质谱（UPLC-MS/MS）同时测定食品中6种非食用色素碱性橙Ⅱ、罗丹明B、苏丹红Ⅰ~Ⅳ的方法。针对不同基质样品采用乙腈或乙腈-水萃取、低温冷冻、C₁₈净化、液相分离后多反应离子监测模式（MRM）测定。对红酒、葵花籽、糖果的检测结果表明，6种非食用色素的方法检出限为1.0~20.0 μg/kg；回收率为72.4%~114.7%，相对标准偏差（RSD）为3.7%~11.3%。与传统前处理方法相比，该方法在有机试剂消耗量、时间上均可节省50%以上。

An improved QuEChERS method is developed for the extraction and purification of food samples. The simultaneous determination method of six inedible pigments including basic orange Ⅱ, Rhodamine B, Sudan red I, Ⅱ, Ⅲ and IV in the samples are simultaneously determined by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) is also established. The samples with different matrix are extracted by acetonitrile or acetonitrile-water, then frozen in refrigerator, purified by C₁₈ and separated by UPLC, finally detected by mass spectrometry in the mode of multriple reaction monitoing (MRM). The detection results on red wine, sunflower seeds and candy show that the detection limits for six inedible pigments by this method varies from 1.0 to 20 μg·kg^-1, the average recovery rates range from 72.4% to 114.7%, the relative standard deviations are in the range of 3.7% to 11.3%. Compared with the traditional pretreatment method, this method can save more than 50% of organic reagents usage and operation time.

曾凯(1983-),男,博士,高级工程师,从事食品质量安全研究,89692176@qq.com。