从自然环境中分离得到一个兼具异养硝化-好氧反硝化(HN-AD)性能和聚集性能的菌群YQS,鉴定其优势属为 Rhizobium、Azoarcu和Hydrogenophaga。综合分析了菌群YQS的脱氮性能、影响异养硝化性能的因素、聚集性能和胞外聚合物(EPS)的分泌情况。结果表明,菌群YQS对氨氮、硝态氮和亚硝态氮的去除率分别为97.33%、89.55%和85.94%;碳源为柠檬酸钠、C/N为12、温度为30℃、转速为130 r/min是菌群YQS进行异养硝化的最佳条件;菌群YQS的良好聚集性能与其分泌的EPS有关,聚集指数可达53.72%。菌群YQS的分离为实现污水生物高效脱氮和功能微生物富集提供了新的生物资源。

A bacterial group YQS with both heterotrophic nitrification-aerobic denitrification (HN-AD) and aggregation properties was isolated from the natural environment,and its dominant genera were identified as Rhizobium,Azoarcu and Hydrogenophaga.The nitrogen removal performance,factors affecting heterotrophic nitrification performance,aggregation performance and extracellular polymer (EPS) secretion of YQS were analyzed comprehensively.The results showed that the removal rates of ammonia nitrogen,nitrate nitrogen and nitrite nitrogen by YQS were 97.33%、 89.55% and 85.94%,respectively.The optimal conditions for heterotrophic nitrification were sodium citrate as carbon source,C/N of 12,temperature of 30℃ and rotation speed of 130 r/min.The good aggregation performance of YQS was related to the secretion of EPS,and the aggregation index reached 53.72%.The isolation of YQS provides a new biological resource for efficient nitrogen removal and functional microbial enrichment in wastewater.

杨欣雨(1998-),女,硕士生,研究方向为水污染控制及资源化利用,1344672130@qq.com;刘玉香(1969-),女,博士,教授,硕士生导师,研究方向为污染物资源化利用与新型生物处理技术的研发,通讯联系人,yuxiangliu2002@126.com。