以2-羟基-1-萘甲醛、邻氨基苯甲醛为原料,设计合成了一种新型Schiff碱Al³⁺荧光探针S,并通过 ¹HNMR、¹³CNMR、ESI-MS、IR对探针S进行结构表征。光谱实验结果表明,探针S在DMSO∶H₂O=4∶1(V/V)体系中,对Al³⁺有良好的荧光和裸眼识别。Al³⁺浓度在2.0~50 μmol/L范围内,体系荧光强度呈现出良好的线性关系,检出限为0.033 μmol/L,探针S与Al³⁺形成1∶1的配合物。通过荧光光谱研究了荧光探针S-Al³⁺配合物与DNA的相互作用,实验结果表明,探针S-Al³⁺配合物对DNA的猝灭过程为动态猝灭,同时对不同温度下相互作用的结合点常数、结合位点数及热力学参数进行了计算,推断二者作用力的类型为疏水作用,作用方式为嵌插作用。

A novel Schiff base fluorescence probe (S) for Al³⁺ is designed and synthesized from 2-hydroxy-1-naphthaldehyde and o-aminobenzaldehyde,and characterized by means of ¹HNMR spectrum,¹³CNMR spectrum,ESI-MS and IR spectrum.The results from spectral analysis show that probe S exhibits good selectivity and naked eye identification to Al³⁺ in a DMSO-H₂O(V/V=4∶1) solution.The concentration of Al³⁺ in the range of 2.0-50 μmol·L^-1 shows a linear relationship with the fluorescent intensity,while the detection limit is 0.033 μmol·L^-1.A complex is formed between the probe S and Al³⁺ in a 1∶1 ratio.The interaction between probe S-Al³⁺ complex and DNA is investigated through fluorescence spectroscopies.Experimental results show that the complex quenches DNA dynamically.In addition,the binding constant,binding-site numbers and thermodynamic parameters of the interaction between probe S-Al³⁺ complex and DNA are calculated at different temperature.It is indicated that the interacting forces belongs to hydrophobic action,showing an intercalation interaction mode.

孟德素(1978-),女,硕士,副教授,研究方向为有机合成及光谱分析,desumeng@126.com