以典型聚集诱导发光（AIE）性质的四苯乙烯基为荧光母体，通过酯基连接上水溶性佳的聚乙二醇基合成了具有AIE特性的化合物TPE-PEG₂₀₀₀-TPE；利用光谱法研究探针TPE-PEG₂₀₀₀-TPE的光学性能，结果表明，探针在DMF/H₂O混合溶液中具有典型的AIE性能，其溶液（探针溶液浓度为10 μmol/L，DMF/H₂O体积比为1/4，在PBS缓冲溶液中pH值为7.4）对ONOO^-表现出"turn-off"特异性识别，猝灭比可达97.86%，检出限为1.26×10^-6 mol/L，具有较好的选择性、较高的灵敏度和较大的Stokes位移（164 nm）。通过薄层色谱法和高分辨质谱法对探针识别ONOO^-的机制进行研究，结果表明，该探针通过酯基水解生成TPE-COOH，进而使荧光发生变化，实现对ONOO^-的特异性识别。

TPE-PEG₂₀₀₀-TPE, a compound with aggregation-induced luminescence (AIE) characteristics, is synthesized by using tetraphenylethylene group with typical AIE property as the fluorescent parent through the ester group connecting water-soluble polyethylene glycol group.The optical properties of TPE-PEG₂₀₀₀-TPE probe are studied by means of spectroscopy, and it is shown that TPE-PEG₂₀₀₀-TPE probe has a typical AIE performance in a DMF/H₂O mixed solution.The solution of TPE-PEG₂₀₀₀-TPE probe (10 μmol/L, v/v, 1/4, PBS, pH=7.4) shows a "turn-off" specific recognition to ONOO^-, with a quenching ratio of 97.86% and a detection limit of 1.26×10^-6 mol·L^-1, and also presenting a better selectivity, a relatively high sensitivity and a large Stokes displacement (164 nm).It is indicated through exploring the recognition mechanism of the probe for ONOO^- by TLC and ESI-MS that this probe generates TPE-COOH through ester group hydrolysis, which enables specific recognition of ONOO^-.

何潞潞(1998-),女,硕士生。