通过对石油烃降解菌Paenarthrobacter ureafaciens Thp2-23进行16S rRNA基因测序分析，利用MEGA 7.0构建菌株系统发育树，并采用二苯碳酰二肼分光光度法检测Thp2-23在不同条件下对Cr （Ⅵ）的还原率；采用SPSS 25.0对各组还原率进行统计学描述及方差分析，探讨pH、温度、石油烃、吐温80和铜离子对菌株还原Cr （Ⅵ）效果的影响。结果表明，菌株Thp2-23在pH 8.0、40℃的最优还原条件下，12 h对50 mg/L Cr （Ⅵ）的还原率为95%，24 h对Cr （Ⅵ）的还原率达到100%。石油烃和吐温80可抑制Thp2-23对Cr （Ⅵ）的还原，铜离子可促进Thp2-23对Cr （Ⅵ）的还原。

A 16S rRNA gene sequencing analysis is done on Paenarthrobacter ureafaciens Thp2-23, a petroleum hydrocarbon degradation strain.The strain phylogenetic tree is constructed by using MEGA 7.0.Diphenylcarbazide spectrophotometric method is employed to detect the reduction rate of Cr(Ⅵ) by Thp2-23 under different conditions.By means of SPSS 25.0 software, descriptive statistical analysis and variance analysis are performed on the reduction rates of each group.The influence of pH, temperature, petroleum hydrocarbons, Tween-80 and copper ion on the effectiveness of Cr(Ⅵ) reduction by the strain is explored.The results indicate that under the optimal reduction conditions including a pH of 8.0 and 40℃, the reduction rate of 50 mg·L^-1 Cr(Ⅵ) by Thp2-23 strain reaches 95% within 12 h, and 100% within 24 h.Cr(Ⅵ) reduction may be inhibited by petroleum hydrocarbon and Tween 80, but promoted by copper ion.

王广顺(1997-),男,硕士生,主要从事环境污染物的微生物修复技术研究,WGS13860163044@163.com