针对污泥厌氧消化水解阶段有机物释放受限、影响产甲烷的问题,通过优化游离氨预处理的剂量,考察了不同游离氨预处理浓度、持续时间下,污泥水解阶段的有机物释放效果。结果表明,与控制组相比,优化剂量的游离氨处理后TCOD、SCOD质量浓度分别提高了134.64%~250.34%、154.70%~274.55%,表明优化的游离氨水平有效强化了污泥的预处理效果。组分分析结果表明,溶解性蛋白质、多糖浓度分别提高111.70%、412.37%,VS水解率增加16.60%~80.38%。三维荧光光谱(3D-EEM)的PARAFAC分析表明,游离氨预处理后提高了生物可降解有机物占比,主要以溶解性微生物副产物(41.18%)、类酪氨酸(29.32%)为主;同时促进难降解有机物的转化。不同于高能耗预处理的强力溶胞破壁,以温和调节的游离氨预处理方式强化水解,可提高液相中溶解性有机物质量浓度,进而改善污泥中有机物释放效果。

In order to solve the problems of limited release in sludge anaerobic digestion hydrolysis stage and affecting methane production,the dosage of free ammonia for pretreatment is optimized,and the release effect of organic matters in sludge hydrolysis stage is investigated under different concentration and duration of free ammonia pretreatment.It is shown that compared with the control group,the mass concentrations of TCOD and SCOD increase by 134.64%-250.34% and 154.70%-274.55%,respectively after the dosage of free ammonia is optimized,meaning that the optimized free ammonia level enhances the pretreatment effect of sludge effectively.The results from component analysis show that the concentrations of soluble protein and polysaccharide increase by 111.70% and 412.37%,respectively,and the hydrolysis rate of VS increases by 16.60%-80.38%.PARAFAC analysis based on 3D fluorescence spectroscopy shows that the pretreatment by free ammonia increases the proportion of biodegradable organic matters,which mainly include soluble microbial byproducts (41.18%) and tyrosines (29.32%).Meanwhile,the conversion of refractory organic matter is promoted.Different from pretreatment by high energy consumption method such as strong cell lysis and wall breaking,the hydrolysis is enhanced by gently regulated free ammonia pretreatment to improve the mass concentration of dissolved organic matters in the liquid phase,thus improving the release effect of organic matters in sludge.

沈嘉辉(1996-),男,硕士研究生,主要研究方向为可再生能源开发利用,18833020604@163.com。