针对土霉素菌渣存在危险处置及不合理利用等问题,采用酶解法制备了土霉素菌渣吸附剂,以实现菌渣的资源化利用。采用高温高压蒸汽预处理菌渣,破坏菌丝体细胞壁和细胞膜结构;采用风味蛋白酶对菌丝体进行酶解处理,使菌丝体中的蛋白质、多肽等成分水解溶出,保留细胞多糖框架结构用作吸附剂的制备。结果表明,菌渣经酶解处理后菌丝分支变短、透光率提高;主要官能团没有发生改变;制备的菌渣吸附剂对亚甲基蓝的吸附率达到78.29%,比原菌渣的吸附量提高了1.44倍,达到了传统吸附剂活性炭的91.14%。

In order to solve the problems of dangerous disposal and irrational utilization of oxytetracycline residue,the adsorbent based on oxytetracycline residue is prepared through enzymatic hydrolysis method.Oxytetracycline residue is pretreated with high temperature and high pressure steam to destroy mycelium cell wall and cell membrane structure.Then the mycelium is treated by flavourzyme to hyrolyze and dissolve the protein and polypeptide in it,and retain cell polysaccharide framework for the preparation of adsorbent.Results show that the branches of mycelium become shorter,and the mycelium improve its transmittance after enzymatic hydrolysis.Major functional groups remain unchanged.The adsorption rate of methylene blue by the prepared oxytetracycline residue absorbent reaches 78.29%,which is1.44 times that by the original oxytetracycline residue,and 91.14% that by traditional activated carbon adsorbent.

杜欣欣(1996-),女,硕士研究生,研究方向为生物工程及酶学研究,857805832@qq.com。